Mechanism and structural dynamics of translation

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The proposed research focuses on the structural and functional dynamics of the cellular machinery that is responsible for the translation of genetic information into functional proteins, a process called translation. Translation takes place at the ribosome, a large macromolecular complex, which in a complex and precisely timed fashion interacts with several additional protein factors to achieve the fast and efficient protein synthesis observed in the living cell. To study the functional and structural dynamics of translation factors on the ribosomes; a purified in vitro translation system will be studied applying a multidisciplinary approach combining techniques ranging form classical biochemistry and fluorescence spectroscopy, to computational techniques such as molecular dynamics calculations.