Posttranslational modification-specific proteomics approach to identification of molecular biomarkers for wheat seed dormancy control and regulation

点击翻译按钮获取中文摘要

Pre-harvest sprouting (PHS) is a perpetual threat to wheat production. Sprouting in wheat can result in massive harvest losses of up to 40% in the price/tonne for producers because sprouted grain is an important downgrading factor. Seed dormancy, which is controlled by genetic and environmental factors, should be strong enough to inhibit germination. PHS resistance is a form of grade protection that is best built into wheat cultivars through genetic resistance. However, still very little is known about the processes and molecular signalling mechanisms occurring during dormancy control. The research program aims to examine sources of wheat seed dormancy and sprouting resistance. I propose the proteomic analysis of dormancy in all aspects - biomarker pattern linking genome to proteome, genetics factors control to specific phenotypic expression plasticity, post-translational signalling, phytohormone-mediated response and effect of environmental changes. In the next five years, in-depth systematic analysis of differential protein correlation profiling including localization, dynamics, and interaction partners will be focused on specific and functionally relevant 'sub-proteomes'. The hybrid lines of doubled haploid populations segregating for dormancy/sprouting resistance will be used to validate the identified candidate biomarker proteins. The research will create new knowledge regarding the biochemical nature of sprouting resistance and dormancy control, will lead to understanding of the biochemical nature for reduced wheat seed dormancy under unfavourable environmental conditions and identify molecular basis and genetic resources for sustained, internationally competitive, variety development. The outcome of this research is the thorough evaluation of germplasm enhanced for sprouting resistance by the systematic proteomic analysis of dormancy biomarkers. The broad basis of the germplasm, derived from elite Canadian genetics, ensures genetic diversity for long term selection as well as for immediate variety development.