Posttranslational modification-specific proteomics approach to identification of molecular biomarkers for wheat seed dormancy control and regulation

小麦穗发芽是小麦生产的一个长期威胁。小麦发芽可能会导致生产者每吨价格损失高达40%，因为发芽谷物是一个重要的降级因素。种子休眠受遗传和环境因素的控制，需要足够的强度来抑制萌发。PHS抗性是一种等级保护形式，最好通过遗传抗性构建到小麦品种中。然而，仍然知之甚少的过程和分子信号发生在休眠控制机制。该研究计划旨在研究小麦种子休眠和发芽抗性的来源。我建议从各个方面对休眠进行蛋白质组学分析--将基因组与蛋白质组联系起来的生物标志物模式、遗传因素对特定表型表达可塑性的控制、翻译后信号传导、植物激素介导的反应以及环境变化的影响。在未来五年内，深入系统的分析差异蛋白质相关性分析，包括本地化，动力学和相互作用的合作伙伴将集中在特定的和功能相关的“亚蛋白质组”。抗休眠/发芽分离的双单倍体群体的杂交系将用于验证所鉴定的候选生物标志物蛋白。这项研究将创造关于发芽抗性和休眠控制的生物化学性质的新知识，将导致对不利环境条件下减少小麦种子休眠的生物化学性质的理解，并确定可持续的、具有国际竞争力的品种开发的分子基础和遗传资源。本研究的成果是通过对休眠生物标志物的系统蛋白质组学分析，对抗穗发芽种质进行了全面的评价。来自加拿大精英遗传学的种质资源的广泛基础，确保了长期选择以及直接品种开发的遗传多样性。

Pre-harvest sprouting (PHS) is a perpetual threat to wheat production. Sprouting in wheat can result in massive harvest losses of up to 40% in the price/tonne for producers because sprouted grain is an important downgrading factor. Seed dormancy, which is controlled by genetic and environmental factors, should be strong enough to inhibit germination. PHS resistance is a form of grade protection that is best built into wheat cultivars through genetic resistance. However, still very little is known about the processes and molecular signalling mechanisms occurring during dormancy control. The research program aims to examine sources of wheat seed dormancy and sprouting resistance. I propose the proteomic analysis of dormancy in all aspects - biomarker pattern linking genome to proteome, genetics factors control to specific phenotypic expression plasticity, post-translational signalling, phytohormone-mediated response and effect of environmental changes. In the next five years, in-depth systematic analysis of differential protein correlation profiling including localization, dynamics, and interaction partners will be focused on specific and functionally relevant 'sub-proteomes'. The hybrid lines of doubled haploid populations segregating for dormancy/sprouting resistance will be used to validate the identified candidate biomarker proteins. The research will create new knowledge regarding the biochemical nature of sprouting resistance and dormancy control, will lead to understanding of the biochemical nature for reduced wheat seed dormancy under unfavourable environmental conditions and identify molecular basis and genetic resources for sustained, internationally competitive, variety development. The outcome of this research is the thorough evaluation of germplasm enhanced for sprouting resistance by the systematic proteomic analysis of dormancy biomarkers. The broad basis of the germplasm, derived from elite Canadian genetics, ensures genetic diversity for long term selection as well as for immediate variety development.