Robotic spot excision system for proteome analysis

点击翻译按钮获取中文摘要

In the last several years proteomics methods have become a vital component of biochemical and biological research, having made possible the identification and characterization of individual proteins and the identification of hundreds to thousands of proteins in high-throughput proteomics experiments. One of the key research methods in this field is 2D gel electrophoresis, which separates a complex mixture of proteins into isolated protein spots on a gel, which may then be excised from the gel and identified by mass spectrometry. This system will permit rapid and precise automated excision of protein spots from a 2D gel with minimal contamination from outside sources, increasing the accuracy and throughput of proteomics experiments. Along with the existing 2D differential in-gel electrophoresis and mass spectrometry capability at the University of Waterloo, this system would complete a proteomics workflow system at UW.