Differential refractometer detector

申请人已经开展了一项研究计划，旨在调查开发功能成分和营养食品的创新生物催化方法。在短期内，申请人计划的主要重点是开发新的酶促糖基化策略，以有效合成非消化低聚糖，其在支持肠道健康和降低癌症风险方面的好处正日益得到承认。新型低聚果糖(FOSS)以不同的戊糖或多元醇取代葡萄糖基，已被确定为潜在的候选者。可以想象，这些新的FOSS可能具有新的免疫调节能力和对病原体的抗黏附活性，这是当前一代FOS所没有的。尽管左旋蔗糖酶(LSS)具有催化合成新型FOSS的潜力，但它们的可用性和操作稳定性仍然受到限制。最初的努力集中在从嗜热微生物来源中诱导和回收新的LSS。为了为更好地调节LSS的作用提供合理的基础，拟议的研究工作的一个重要部分将依赖于对这些酶发挥作用的机制的调查。控制LS催化的果糖转移的热力学平衡的驱动力也将被研究，以便更好地合成定义明确的FOSS。为了能够方便地调节转移果糖基化过程，将研究LSS在纳米材料载体上的固定化，强调纳米材料的特性与LSS效率之间的关系。进一步探索固定化和分子印迹的合理结合，以提高LS的选择性。生产的自由/开源软件将根据它们的结构和生物活性进行表征。所要求的折射仪探测器是这一计划的关键，将与通过CIF-LOF赠款购买的高效液相系统相结合。这种检测设备用于对转果糖基化产物进行定量分析，并对其进行提纯，以便通过核磁共振和傅立叶变换红外光谱进行进一步的结构分析。

The applicant has undertaken a research program aimed at investigating innovative biocatalytic approaches toward the development of functional ingredients and nutraceuticals. In the short term, the primary focus of the applicant's program is on the development of novel enzymatic glycosylation strategies for efficient synthesis of non-digestible oligosaccharides whose benefits, in supporting intestinal health and reducing the risk of cancers, are being increasingly recognized. Novel fructooligosaccharides (FOSs), headed with different pentoses or polyols instead of a glucosyl moiety, have been identified as potential candidates. These novel FOSs may conceivably possess new immunomodulatory capacity and antiadhesive activity against pathogens that are not present in the current FOS generation. Although levansucrases (LSs) are being increasingly recognized for their potential to catalyze the synthesis of novel FOSs, their availability and their operational stability are still limited. Initial efforts have been focused on the induction and the recovery of novel LSs from thermophilic microbial sources. To provide a rational basis for a better modulation of the actions of LSs, an important part of the proposed research work will rely on the investigation of the mechanism by which these enzymes function. The driving forces that control the thermodynamic equilibrium of LS-catalyzed transfructosylation will also be studied for a greater yield synthesis of well-defined FOSs. With the aim of enabling easy modulation of the transfructosylation processes, the immobilization of LSs on nanomaterial supports will be studied, emphasizing the relationships between the features of nanomaterials and the efficiency of LSs. The rational combination of immobilization and molecular imprinting will be further explored to improve the LS selectivity. The produced FOSs will be characterized in terms of their structures and their biological activities. The requested Refractometer detector is key to this program and will be coupled with the HPLC system purchased through the awarded CIF-LOF grant. This detector equipment is needed for the quantitative analysis of the transfructosylation products as well as for their purification for further structural analyses by NMR and FTIR.