Differential refractometer detector

申请人已经承担了一项研究计划，旨在研究创新的生物催化方法，以开发功能性成分和营养保健品。在短期内，申请人项目的主要重点是开发新的酶糖基化策略，以有效合成不可消化的低聚糖，其在支持肠道健康和降低癌症风险方面的益处日益得到认可。新型低聚果糖（FOSs），以不同的戊糖或多元醇代替葡萄糖基部分，已被确定为潜在的候选化合物。这些新型的FOSs可能具有新的免疫调节能力和抗病原体的抗粘附活性，这在当前的FOS中是不存在的。尽管左旋蔗糖酶（LSs）因其催化合成新型FOSs的潜力而日益得到认可，但其可用性和操作稳定性仍然有限。最初的努力集中在从嗜热微生物源诱导和回收新型LSs上。为了为更好地调控LSs的作用提供合理的依据，研究这些酶的作用机制将是拟议研究工作的一个重要组成部分。控制ls催化转果糖基化热力学平衡的驱动力也将被研究，以获得更高收率的合成明确的FOSs。为了使转果糖基化过程易于调节，将研究纳米材料载体上的LSs固定化，强调纳米材料特性与LSs效率之间的关系。将进一步探索固定化与分子印迹的合理结合，以提高LS的选择性。所产生的自由/开源软件将根据其结构和生物活性进行表征。所要求的折射仪探测器是该项目的关键，将与通过授予的CIF-LOF拨款购买的HPLC系统相结合。该检测设备用于对转果糖基化产物进行定量分析，并对其进行纯化，以便通过NMR和FTIR进行进一步的结构分析。

The applicant has undertaken a research program aimed at investigating innovative biocatalytic approaches toward the development of functional ingredients and nutraceuticals. In the short term, the primary focus of the applicant's program is on the development of novel enzymatic glycosylation strategies for efficient synthesis of non-digestible oligosaccharides whose benefits, in supporting intestinal health and reducing the risk of cancers, are being increasingly recognized. Novel fructooligosaccharides (FOSs), headed with different pentoses or polyols instead of a glucosyl moiety, have been identified as potential candidates. These novel FOSs may conceivably possess new immunomodulatory capacity and antiadhesive activity against pathogens that are not present in the current FOS generation. Although levansucrases (LSs) are being increasingly recognized for their potential to catalyze the synthesis of novel FOSs, their availability and their operational stability are still limited. Initial efforts have been focused on the induction and the recovery of novel LSs from thermophilic microbial sources. To provide a rational basis for a better modulation of the actions of LSs, an important part of the proposed research work will rely on the investigation of the mechanism by which these enzymes function. The driving forces that control the thermodynamic equilibrium of LS-catalyzed transfructosylation will also be studied for a greater yield synthesis of well-defined FOSs. With the aim of enabling easy modulation of the transfructosylation processes, the immobilization of LSs on nanomaterial supports will be studied, emphasizing the relationships between the features of nanomaterials and the efficiency of LSs. The rational combination of immobilization and molecular imprinting will be further explored to improve the LS selectivity. The produced FOSs will be characterized in terms of their structures and their biological activities. The requested Refractometer detector is key to this program and will be coupled with the HPLC system purchased through the awarded CIF-LOF grant. This detector equipment is needed for the quantitative analysis of the transfructosylation products as well as for their purification for further structural analyses by NMR and FTIR.