Cryogenic storage of C. elegans stocks for molecular genetic research

长期低温储存设施是我国碳的短期和长期储存的必要资源。elegans菌株、大量收集的fossils、cossils、C. elegans ORFeome文库和有效完整的C.线虫RNAi文库。 C.秀丽隐杆线虫可以保存多年（至少30年），并可回收用于进一步分析，这是C.线虫已被广泛应用于重要的生物医学研究项目和重大生物学发现。 此外，冷冻的菌株不进行DNA复制，从而降低了积累不需要的突变的可能性，当菌株在平板上长时间保存时可能发生这种情况。 三个申请人生产和储存的菌株数量约为13，000个，新菌株不断产生。 自线虫C.秀丽隐杆线虫在20世纪70年代早期被鉴定为模式生物，其本身代表了申请人研究和发现的宝贵资源，以及C.在加拿大和国外的研究人员。在没有足够低温储存的情况下维持这些菌株将是昂贵的、可怕的和危险的，因为许多珍贵的菌株可能会丢失。我们三个研究小组共同申请资金，购买一台超低温冰箱和两个液氮储罐，以取代我们的超低温冰箱，这些冰箱的预期寿命即将结束，并储存新产生的C。elegans菌株和成千上万的分子DNA构建体。 所有三个实验室目前的低温储存设施已接近其容量，此外，失去一个超低温冷冻箱而没有储存设施来转移菌株，将导致多年积累的大量宝贵菌株的损失。

Long term cryogenic storage facilities are an essential resource for short term and long term storage of our C. elegans strains, large collection of fosmids, cosmids, a C. elegans ORFeome library, and an effectively complete C. elegans RNAi library. C. elegans can be stored for many years (at least 30 years) and recovered for further analysis, which has been a key reason why C. elegans has been widely used in important biomedical research projects and major biological discoveries. In addition, frozen strains do not undergo DNA replication thereby lowering a probability of accumulating unwanted mutations as can happen when strains are maintained for long periods on plates. The number of strains generated and stored by the three applicants is on the order of 13,000 with new strains being generated continuously. The large collection of strains, which has been established since the nematode C. elegans was identified as a model organism in the early 1970s, by itself represents a precious resource for research and discovery for the applicants, as well as C. elegans researchers in Canada and abroad. Maintaining these strains without adequate cryogenic storage would be expensive, formidable, and risky since many precious strains could be lost. We three research groups together request funds for an ultra-low temperature refrigerator and two liquid Nitrogen storage tanks to replace our ultra-low temperature refrigerators, which are approaching the end of their expected life-spans, and to store newly generated C. elegans strains and many thousands of molecular DNA constructs. The current cryogenic storage facilities for all three laboratories are filled close to their capacities and in addition, the loss of an ultra-low temperature freezer without a storage facility to move strains to would result in the loss of a large number of valuable strains accumulated over many years.