Replacement for Fast Protein Liquid Chromatography System

纯化蛋白质的能力是大多数生物化学研究的基石。纯化的蛋白质是大多数生物功能的酶、调节剂和伴侣。快速有效地纯化蛋白质的能力为研究酶活性、复合物形成和各种常规生化反应提供了强有力的工具。通过使用快速液相色谱，我们开发了用于纯化几种专门的酶和蛋白质的方法，包括核糖核酸酶III的不同直向同源物，几种RNA结合蛋白以及标准酶如Taq DNA聚合酶和T7 RNA聚合酶。我们已经为舍布鲁克大学的数十名研究人员纯化了数百种蛋白质，并指导了数十名研究生研究蛋白质的结构和功能。该应用程序的目标是取代我们的液相色谱系统，以维护舍布鲁克大学的蛋白质纯化平台作为服务。我们的机构必须保持其提供蛋白质纯化解决方案的能力，以维持NSERC资助的需要蛋白质纯化和分析的研究项目。

The capacity to purify proteins is the corner stone of most biochemical studies. Purified proteins are the enzymes, regulators and chaperone of most biological functions. The ability to quickly and efficiently purify proteins provides powerful tools for studying enzymatic activity, complex formation and a variety of routine biochemical reactions. By using fast liquid chromatography, we developed methods for the purification of several specialized enzymes and proteins including different orthologues of Ribonuclease III, several RNA binding proteins as well as standard enzymes like Taq DNA polymerase and T7 RNA polymerase. We have purified hundreds of proteins for tens of researchers at the Université de Sherbrooke and coached dozens of graduate students in their studies of protein structure and function. The goal of this application is to replace our liquid chromatography system needed to maintain the protein purification platform as a service at Université de Sherbrooke. It is essential for our institution to keep its capacity to provide protein purification solutions in order to maintain the NSERC-funded research programs requiring the purification and analysis of proteins.