Structural, physiological and biochemical aspects of embryo development in vivo and in vitro

体内和体外胚胎发育的结构、生理和生化方面

• 批准号: 6704-2007
• 负责人: Yeung, Edward
• 金额: $ 4.01万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=450017
• 关键词: Structural; physiological; biochemical; aspects; embryo

Apical meristems are located at each growing point and are specialized for the production of new cells for the plant body. The importance of meristems in plant growth and development can be summarized by a simple statement from Professor Ian Sussex's review "The meristems make the plant" (Cell 56: 225, 1989). In the past funding period, we demonstrated that shoot meristem initiation is sensitive to auxin perturbation. Furthermore, during in vitro embryogenesis, although a shoot meristem is formed, it is not stably determined as its organization deteriorates upon prolong culture. The root meristem, on the other hand, requires auxin for development. These observations suggest that expression of the meristem genes and hence the cell fate could be altered by auxin levels and factors from the embryonic environment. We also established that the redox state of both the cell and its surrounding environment can modulate in vitro embryo development. The redox environment can influence the quality of the embryos produced, especially the shoot apical meristem.

顶端分生组织位于每个生长点，专门为植物体生产新细胞。 分生组织在植物生长和发育中的重要性可以通过来自Ian Sussex教授的综述“The meristems make the plant”（Cell 56：225，1989）的简单陈述来概括。在过去的资助期间，我们证明了芽分生组织的启动是敏感的生长素扰动。 此外，在离体胚胎发生过程中，虽然形成了茎分生组织，但由于其组织在长时间培养后劣化，因此不能稳定地确定。另一方面，根分生组织的发育需要生长素。这些观察结果表明，分生组织基因的表达，因此细胞的命运可以改变生长素水平和胚胎环境的因素。我们还确定了细胞及其周围环境的氧化还原状态可以调节体外胚胎发育。 氧化还原环境可以影响产生的胚的质量，特别是茎尖分生组织。