Analysis of endogenous small RNA pathways in C. elegans

线虫内源小 RNA 通路分析

• 批准号: 341579-2007
• 负责人: Rocheleau, Christian
• 金额: $ 2.27万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=458459
• 关键词: Analysis; endogenous; small; RNA; pathways

Units of DNA, genes, are the blueprints that encode for proteins, which are the building blocks and machinery of the cell. Messenger RNA (mRNA) relays the genetic information from gene to protein. Not all genes and their corresponding proteins are active in all cells, rather different cells "express" a different array of genes and proteins. The activity of a particular gene can be regulated at the level of mRNA production or at the level of protein production. It has recently become apparent that new mechanisms involving small RNAs function to regulate gene activity at both the level of mRNA production and mRNA stability. We are just beginning to understand the various mechanisms by which small RNAs can regulate gene activity. We have uncovered a role for small RNAs in regulating a cellular process often disrupted in human cancers, the Ras signal transduction pathway. However, we do not know the target or the mechanism of this small RNA. Our lab will determine how theses small RNAs regulate Ras signaling, as well as potential identify new proteins important for small RNA function and uncover the potentially novel mechanism by which these small RNAs regulate gene activity.

DNA的单位，基因，是编码蛋白质的蓝图，蛋白质是细胞的构建模块和机器。 信使RNA（mRNA）将遗传信息从基因传递到蛋白质。 并不是所有的基因和它们相应的蛋白质在所有的细胞中都是活跃的，而是不同的细胞“表达”不同的基因和蛋白质。 特定基因的活性可以在mRNA产生水平或蛋白质产生水平上调节。 最近已经变得明显的是，涉及小RNA的新机制在mRNA产生和mRNA稳定性水平上调节基因活性。 我们刚刚开始了解小RNA调节基因活性的各种机制。 我们已经发现了小RNA在调节人类癌症中经常被破坏的细胞过程中的作用，即Ras信号转导通路。 然而，我们不知道这种小RNA的靶点或机制。 我们的实验室将确定这些小RNA如何调节Ras信号传导，以及潜在的识别对小RNA功能重要的新蛋白质，并揭示这些小RNA调节基因活性的潜在新机制。