Peptide sequence effects on nanofiber formation, hydration and in-gel protein mobility/activity

肽序列对纳米纤维形成、水合和凝胶内蛋白质迁移率/活性的影响

• 批准号: 355553-2008
• 负责人: Unsworth, Larry
• 金额: $ 1.75万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=459448
• 关键词: Peptide; sequence; effects; nanofiber; formation

Self-assembling peptide hydrogels, composed of nanofibers with nanoscale dimensions, are becoming recognized as a robust platform for a variety of nanomedicine applications: facilitating axon regeneration, sustaining 3D cellular environment, delivering active proteins and small molecules. Preliminary studies indicate a lack of immune response or initiation of the coagulation cascade to these materials: the mechanisms of which remain unknown. These properties, plus the ability to precisely control the peptide sequence, and thereby incorporate cellular and molecular targeting capabilities, makes self-assembled peptide hydrogels very attractive for nanomedicine applications. However, it remains apparent that the peptide hydrogel interaction with a complex biological milieu has yet to be studied in detail. Through understanding the effects of the peptide composition on gel properties as well as protein interactions, it may be possible to develop these materials for multiple technologies: enzymatically controlled drug delivery, tissue engineering scaffolds to enhance wound healing, high surface area biosensors, cardiovascular grafts, etc.

由纳米尺寸的纳米纤维组成的自组装肽水凝胶正在被认为是各种纳米医学应用的强大平台：促进轴突再生、维持3D细胞环境、递送活性蛋白质和小分子。初步研究表明，这些材料缺乏免疫反应或启动凝血级联反应：其机制仍然未知。这些性质，加上精确控制肽序列的能力，从而结合细胞和分子靶向能力，使得自组装肽水凝胶对于纳米医学应用非常有吸引力。然而，很明显，肽水凝胶与复杂的生物环境的相互作用还有待详细研究。通过了解肽组合物对凝胶性质以及蛋白质相互作用的影响，有可能开发用于多种技术的这些材料：酶控药物递送、增强伤口愈合的组织工程支架、高表面积生物传感器、心血管移植物等。