Genetic and physiological basis of growth, survival and reproduction in Chinook salmon: applications for aquaculture

拟议研究的主要目标是开发和评估高性能和低性能的奇努克三文鱼品系，直接或杂交用于加拿大西海岸的有机三文鱼养殖业。因此，这些品系和杂交品种不仅必须具有高生长和繁殖性能，而且还必须具有特殊的抗病能力，因为使用治疗药物是不可能的。我们将以4个主要目标来实现我们的目标：1)利用我们产业合作伙伴黄岛水产养殖有限公司(YIAL)设施中现有的精选Chinook鲑鱼高、低存活率品系进行量化遗传育种实验；2)评估YIAL选育品系以及量化遗传育种实验后代中先前开发的性能遗传标记的状况；3)测量育种实验后代的生活期特定表现(如存活、生长)；4)确定性能性状的遗传基础并估计性状之间的遗传相关性。由于我们将使用无源集成应答器(PIT)标签技术跟踪来自100个家庭组的单个后代，我们将能够确定对水产养殖有价值的特定性状的遗传力和其他可能增加品系性能的遗传参数。这项研究的商业营销潜力很大，无论是在加拿大国内还是出口。拟议的研究不仅将为学术合作伙伴提供与加拿大水产养殖业合作的机会，还将产生应用科学和基础科学、训练有素的学生和科学出版物。

The primary objective of the proposed research is to develop and evaluate high and low performance strains of Chinook salmon for direct or hybrid use in the Organic Salmon Aquaculture industry on the west coast of Canada. The strains and hybrids must therefore not only have high growth and reproductive performance, they must also have exceptional disease resistance, because theraputant use is not possible. We will address our objective with 4 broad goals; 1) use the existing selected high and low survival lines of Chinook salmon at our Industrial partner's (Yellow Island Aquaculture Ltd.; YIAL) facility to carry out a quantitative genetic breeding experiment, 2) evaluate the status of the previously-developed performance genetic markers in the YIAL selected lines as well as in the offspring from the quantitative genetic breeding experiment, 3) measure life-stage specific performance (e.g. survival, growth) in the offspring from the breeding experiment, and 4) determine the genetic basis of performance traits and estimate genetic correlations among traits. Since we will follow individual offspring from 100 family groups using Passive Integrated Transponder (PIT) tag technology, we will be able to determine the heritability for specific traits of value to aquaculture and other genetic parameters that may add performance to the lines. The potential for commercial marketing of this research is good, both within Canada and for export. The proposed research will not only provide an opportunity for the academic partners to collaborate with the Canadian aquaculture industry, it will generate applied and basic science, highly trained students and scientific publications.