Early evolutionary origins of mechanosensory transduction: structure, function and phylogenetic studies of the family of mechanosensitive channels in cell-walled organisms.

机械感觉转导的早期进化起源：细胞壁生物机械敏感通道家族的结构、功能和系统发育研究。拟议的项目是基于申请人最近取得的进展：(i)在细胞壁生物中确定一个新的机械敏感（MS）通道家族，包括古细菌和细菌（原核生物），以及真菌和植物（真核生物），以及（ii）确定MscL结构动力学的基本方面，原型MS通道，使用电子顺磁共振波谱和分子动力学模拟结合膜片钳技术。该项目的目的是阐明质谱通道功能的分子原理，这对于理解整个生物界机械感觉转导的生物物理学和生理学至关重要。

Early evolutionary origins of mechanosensory transduction: structure, function and phylogenetic studies of the family of mechanosensitive channels in cell-walled organisms. The proposed project is based on the recent progress the applicants have made by (i) identifying a novel family of mechanosensitive (MS) channels in cell-walled organisms, which include Archaea and Bacteria (prokaryotes), as well as fungi and plants (eukaryotes), and (ii) determining basic aspects of the structural dynamics of MscL, the prototype MS channel, using electronparamagnetic resonance spectroscopy and molecular dynamics simulations in combination with the patch clamp technique. The aim of the project is to elucidate the molecular principles underlying the function of MS channels, which is of fundamental importance for understanding of the biophysics and physiology of mechanosensory transduction throughout the biological world.