Development of microbiological testing technology for drinking water

在女王大学，我们开发了一种检测水中微生物污染物的新技术。这项工作是对2000年5月沃克顿惨案的直接回应。我们的技术是唯一可以在现场检测大肠杆菌和总大肠菌群的技术，并且比现有的检测方法具有更高的可靠性。这有可能大大提高加拿大和全球范围内的水安全和监测能力。病原体检测系统公司（PDS）于2003年在金斯敦成立，旨在将该技术商业化。在这项建议中，我们建议与PDS合作，以提高技术，并大大扩大可检测污染物的范围。今天的技术状况总结如下：i) EC和TC的测试给出了“存在/不存在”（P/A）结果，可作为原型，组合测试在台式手动采样仪器的一次性（一次性）测试盒中运行。ii) P/A结果已被认证并批准在安大略省使用（见下文）。iii)检测需要18小时才能宣布“缺失”结果，阳性样本（少至一个细胞）通常在14小时内检测到。在建议的工作周期结束时，我们将完成以下技术：1)更快的EC和TC测试，通常在10-12小时内检测到单个生物体，“缺席”时间为14-16小时；以及更快速的EC-only测试，在8-10小时内检测到单个生物体。2)定量测试能力，其中“群体形成单位当量”（CFU）值与P/ a结果一起报告。3)一个

At Queen's we have developed a new technology for microbiological contaminant detection in water. This work was initiated in direct response to the Walkerton tragedy of May 2000. Ours is the only technology which can test for E. coli and Total Coliform bacteria on-site and perform with higher reliability than current tests. This has the potential to dramatically improve water safety and monitoring capacity in Canada and on a global scale. The company Pathogen Detection Systems (PDS) was formed in Kingston in 2003 to commercialize this technology. In this proposal, we propose to partner with PDS to enhance the technology and to significantly expand the range of contaminants which can be detected. The state of the technology today is summarized as follows: i) A test for EC and TC giving a "presence/absence" (P/A) result is available as a prototype, with the combined test running in disposable (single-use) test cartridges in a bench-top, manual sampled instrument. ii) The P/A result has been certified and approved for use in Ontario (see below). iii) The test takes 18 hrs to declare an "absent" result, with positive samples (as few as one cell) normally detected in under 14 hrs. At the end of the proposed work period, we will have accomplished the following to the technology: 1) A faster EC and TC test, with single organisms normally detected in 10-12 hrs and an "absent" time of 14-16 hrs, and a more rapid EC-only test with single organisms detected in 8-10 hrs. 2) Quantitative testing capability, where a "colony forming unit-equivalent" (CFU) value is reported with The P/A result. 3) A