Development of microbiological testing technology for drinking water

在Queen's，我们开发了一种新技术，用于检测水中的微生物污染物。这项工作是针对2000年5月的沃克顿悲剧而发起的。我们的技术是唯一可以检测E。大肠杆菌和总大肠菌群在现场和执行更高的可靠性比目前的测试。这有可能大大提高加拿大和全球范围内的水安全和监测能力。病原体检测系统公司（PDS）于2003年在金斯顿成立，旨在将该技术商业化。在本提案中，我们建议与PDS合作，以增强技术并显着扩大可检测的污染物范围。目前的技术状况总结如下：i）给出“存在/不存在”（P/A）结果的EC和TC测试可作为原型，其中组合测试在台式手动采样仪器中的一次性（一次性）测试盒中运行。ii）P/A结果已被认证并批准在安大略使用（见下文）。iii）测试需要18小时才能宣布“不存在”结果，阳性样品（少至一个细胞）通常在14小时内检测到。在建议的工作期结束时，我们将完成以下技术：1）更快的EC和TC测试，通常在10-12小时内检测到单个微生物，14-16小时的“缺席”时间，以及更快的仅EC测试，在8-10小时内检测到单个微生物。2)定量检测能力，其中“菌落形成单位当量”（CFU）值与P/A结果一起报告。3)一

At Queen's we have developed a new technology for microbiological contaminant detection in water. This work was initiated in direct response to the Walkerton tragedy of May 2000. Ours is the only technology which can test for E. coli and Total Coliform bacteria on-site and perform with higher reliability than current tests. This has the potential to dramatically improve water safety and monitoring capacity in Canada and on a global scale. The company Pathogen Detection Systems (PDS) was formed in Kingston in 2003 to commercialize this technology. In this proposal, we propose to partner with PDS to enhance the technology and to significantly expand the range of contaminants which can be detected. The state of the technology today is summarized as follows: i) A test for EC and TC giving a "presence/absence" (P/A) result is available as a prototype, with the combined test running in disposable (single-use) test cartridges in a bench-top, manual sampled instrument. ii) The P/A result has been certified and approved for use in Ontario (see below). iii) The test takes 18 hrs to declare an "absent" result, with positive samples (as few as one cell) normally detected in under 14 hrs. At the end of the proposed work period, we will have accomplished the following to the technology: 1) A faster EC and TC test, with single organisms normally detected in 10-12 hrs and an "absent" time of 14-16 hrs, and a more rapid EC-only test with single organisms detected in 8-10 hrs. 2) Quantitative testing capability, where a "colony forming unit-equivalent" (CFU) value is reported with The P/A result. 3) A