Prostaglandin elisa assay system

前列腺素酶联免疫吸附测定系统

• 批准号: 406123-2011
• 负责人: Fortier, Michel
• 金额: $ 1.2万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=472391
• 关键词: Prostaglandin; elisa; assay; system

Endocrinology of female reproduction is the general research program conducted in my laboratory. Recognition and establishment of pregnancy in ruminants (cattle) is the most important component and has been funded by NSERC GDP for 26 years. Prostaglandins are key regulators of female reproductive function and particularly involved in the communication between the uterus and the ovary to regulate the duration of the estrous cycle. We have proposed that PGE2 and PGF2a rather than PGF2a alone determines luteolysis or maintenance of the CL at the time of recognition of pregnancy. In the present grant cycle, we will confirm the role of our newly identified PGF synthase AKR1B1, its regulation and feed-back interaction with PGE synthases to effect a tight control of the PGE/PGF ratio. We rely on an in house Elisa assay for determination of .Prostaglandins E2 and F2a from cell and explants cultures. Until now and for more than 20 years, we have been relying on borrowed equipment for our assays with obvious limitation on priority of use and maintenance of proper condition. The equipment on which we rely presently is more than 20 years old and often out of order while repair parts are difficult to obtain. Moreover, our laboratory is going to move in another building and we will be far from this daily used strategic equipment. The present request is for a new integrated system including all components to complete an Elisa assay; plate washing, reading and standard curve plotting for data analysis.

女性生殖内分泌学是我实验室进行的一般研究项目。反刍动物（牛）妊娠的识别和建立是最重要的组成部分，26年来一直由NSERC GDP资助。前列腺素是女性生殖功能的关键调节剂，特别是参与子宫和卵巢之间的通信，以调节发情周期的持续时间。我们提出，PGE 2和PGF 2a，而不是PGF 2a单独决定黄体溶解或维持CL时，承认怀孕。在本资助周期中，我们将确认我们新鉴定的PGF合酶AKR 1B 1的作用，其调节和与PGE脱氢酶的反馈相互作用，以实现对PGE/PGF比率的严格控制。我们依靠内部Elisa测定来测定来自细胞和外植体培养物的前列腺素E2和F2 a。到目前为止，20多年来，我们一直依赖借来的设备进行分析，在使用优先权和维护适当条件方面存在明显限制。我们目前所依赖的设备已经使用了20多年，经常出现故障，而且很难获得维修零件。此外，我们的实验室将搬到另一座大楼，我们将远离这些日常使用的战略设备。目前的要求是一个新的集成系统，包括所有组件，以完成ELISA测定;平板洗涤，阅读和标准曲线绘制数据分析。