Prostaglandin elisa assay system

女性生殖内分泌学是我实验室进行的一般研究项目。反刍动物（牛）怀孕的识别和建立是最重要的组成部分，已经由NSERC GDP资助了26年。前列腺素是女性生殖功能的关键调节因子，特别是参与子宫和卵巢之间的交流，以调节发情周期的持续时间。我们已经提出PGE2和PGF2a而不是单独的PGF2a决定黄体溶解或维持妊娠时的CL。在目前的资助周期中，我们将确认我们新发现的PGF合成酶AKR1B1的作用，它与PGE合成酶的调节和反馈相互作用，以实现PGE/PGF比例的严格控制。我们依靠室内Elisa法来测定。细胞和外植体培养的前列腺素E2和F2a。到目前为止，20多年来，我们一直依靠借来的仪器进行检测，在优先使用和维护条件方面存在明显的局限性。我们目前所依赖的设备已有20多年的历史，经常出现故障，而维修零件又难以获得。此外，我们的实验室将搬到另一栋楼，我们将远离这些日常使用的战略设备。目前的要求是一种新的集成系统，包括完成Elisa检测的所有组件；洗板，读数和标准曲线绘制数据分析。

Endocrinology of female reproduction is the general research program conducted in my laboratory. Recognition and establishment of pregnancy in ruminants (cattle) is the most important component and has been funded by NSERC GDP for 26 years. Prostaglandins are key regulators of female reproductive function and particularly involved in the communication between the uterus and the ovary to regulate the duration of the estrous cycle. We have proposed that PGE2 and PGF2a rather than PGF2a alone determines luteolysis or maintenance of the CL at the time of recognition of pregnancy. In the present grant cycle, we will confirm the role of our newly identified PGF synthase AKR1B1, its regulation and feed-back interaction with PGE synthases to effect a tight control of the PGE/PGF ratio. We rely on an in house Elisa assay for determination of .Prostaglandins E2 and F2a from cell and explants cultures. Until now and for more than 20 years, we have been relying on borrowed equipment for our assays with obvious limitation on priority of use and maintenance of proper condition. The equipment on which we rely presently is more than 20 years old and often out of order while repair parts are difficult to obtain. Moreover, our laboratory is going to move in another building and we will be far from this daily used strategic equipment. The present request is for a new integrated system including all components to complete an Elisa assay; plate washing, reading and standard curve plotting for data analysis.