Upgrade for an AB3130 DNA genetic analyzer / Mise à niveau d'un analyseur de fragment AB3130

本提案的目的是获得DNA遗传分析仪（AB 3130）的升级，该分析仪代表了我们在舍布鲁克大学分子生态设施的核心。近年来，我们的部门发展迅速，导致需要进行遗传分析的研究人员数量显著增加。因此，在实际配置下，我们的基因分析仪是全时使用的，其处理能力已经不能满足我们的研究需要。由于用户的等待名单很长，我们的分析出现了延迟，这成为获得尽可能高质量数据的主要制约因素。升级到AB3130xl配置将解决这个问题，并有助于将遗传分析仪设置为生物系的多用户，跨学科和集中设施。该基础设施将支持五个学院、他们的研究团队以及他们的合作者的基因分型和测序分子应用。这次升级将满足我们数年的分析能力需求。所要求的设备还将允许我们的实验室继续招聘和培训本科生研究助理，研究生和博士后研究员，以开展我们的研究项目。在未来5年内，至少有50名HQP应接受使用该设备的培训。这种培训将提高他们在私营和公共部门获得高质量工作的机会。总之，DNA基因分析仪的升级是一项基本需要的设备，我们的研究完全依赖于该设备的获得，以保持在国际水平上的生产力和竞争力。

The objective of this proposal is to obtain an upgrade for a DNA genetic analyzer (AB 3130) that represents the core of our molecular ecology facility at Sherbrooke University. Our department has grown rapidly over recent years, resulting in a significant increase in the number researchers needing to conduct genetic analyses. As a result, under its actual configuration, our genetic analyzer is used full time and its processing capacity does not meet our research needs anymore. We are getting delays in analyses because of the long waiting list of users and this becomes a major constraint on obtaining the highest quality of data as possible. An upgrade to an AB3130xl configuration will solve this problem and help to set-up the genetic analyzer as a multi-user, interdisciplinary and centralized facility in the Department of biology. The infrastructure will support genotyping and sequencing molecular applications for five Faculty, their research teams as well as their collaborators. This upgrade will satisfy our needs for analytical capacities for several years. The requested equipment will also allow our laboratories to maintain the recruitment and training of undergraduate research assistants, graduate students and postdoctoral fellows to work on our research programs. At least 50 HQP should be trained using this equipment over the next 5 years. Such training will improve their chances of obtaining a job of high quality in both private and public sectors. In summary, the upgrade for the DNA genetic analyzer is a fundamentally needed piece of equipment and our research is completely dependent on the acquisition of this equipment to remain productive and competitive at the international level.