A droplet visualization system for lab-on-a-chip applications

用于芯片实验室应用的液滴可视化系统

• 批准号: 406855-2011
• 负责人: Ren, Carolyn
• 金额: $ 8.3万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=472572
• 关键词: droplet; visualization; system; lab; chip

The proposed research aims to develop droplet-based microfluidic technology for high-throughput combinatorial studies which are critical to drug discovery and disease diagnosis applications. The majority of microfluidic chips are based on single phase systems consisting of various miscible aqueous streams, which has several limitations, particularly for large-scale combinatorial studies in chemistry, proteomics, genomics, drug screening and biology because it is plagued with cross-contamination between streams. Such a system also scales up poorly as it requires an approximately linear increase in active components and device footprint for each additional reactor. Two-phase droplet-based microfluidics is a viable alternative where discrete droplets are formed from immiscible fluids like water and oil. Each droplet is essentially an ideal microreactor with a specified volume that compartmentalizes reagents preventing them from diffusing away. Microfluidic droplets can scale up performance effectively as they can be generated at rates between 1-20 kHz over a range of picolitres to nanolitres with a standard deviation of only 1-3 %.

拟议的研究旨在开发基于液滴的微流控技术，用于高通量组合研究，这对药物发现和疾病诊断应用至关重要。大多数微流控芯片基于由各种可混溶的水流组成的单相系统，这具有若干限制，特别是对于化学、蛋白质组学、基因组学、药物筛选和生物学中的大规模组合研究，因为它受到流之间的交叉污染的困扰。这样的系统也很难按比例放大，因为它需要每个附加反应器的有源部件和装置占地面积的近似线性增加。基于两相液滴的微流体是一种可行的替代方案，其中离散液滴由不混溶的流体如水和油形成。每个液滴本质上是一个理想的微反应器，具有特定的体积，将试剂分隔开来，防止它们扩散。微流体液滴可以有效地放大性能，因为它们可以在皮升到纳升的范围内以1-20 kHz之间的速率产生，标准偏差仅为1- 3%。