Role of activating Protein 1 in skeletal myogenesis

激活蛋白 1 在骨骼肌生成中的作用

• 批准号: 154249-2011
• 负责人: McDermott, John
• 金额: $ 2.19万
• 依托单位: York University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=475953
• 关键词: Role; activating; Protein; skeletal; myogenesis

In the proposed work we are attempting to understand the events underlying the development of cells that give rise to skeletal muscle in mammals. The commitment of cells to become specialized as muscle cells requires a particular set of genes to be expressed which are required for these cells to be able to have specialized functions such as being able to contract (allowing movement), to respond to nerve impulses from the nervous system (voluntary control of movement) and to function as a depot for the storage of glucose (metabolic control). In order to regulate the expression of these "muscle-specific" genes there are proteins in the nucleus called transcription factors which determine which genes are expressed (transcribed). These proteins function by directly binding to control regions in DNA. In our studies we are particularly interested in a protein called Fra2 which functions in this capacity as a regulator of transcription. Our studies to date indicate that Fra2 may be an important part of a network of transcription factors controlling the formation of skeletal muscle. Our studies are thus aimed at understanding how Fra2 regulates transcription in association with other factors, and also which genes Fra2 specifically targets in the genome. We will therefore use state of the art approaches in genome analysis to elucidate the function of the Fra2 protein in muscle cells.

在拟议的工作中，我们试图了解哺乳动物骨骼肌细胞发育的基础事件。细胞分化为肌细胞需要表达一组特定的基因，这些基因是这些细胞能够具有特定功能所必需的，例如能够收缩（允许运动），对来自神经系统的神经冲动做出反应（自主控制运动）以及作为葡萄糖储存库（代谢控制）。为了调节这些“肌肉特异性”基因的表达，细胞核中存在称为转录因子的蛋白质，其决定哪些基因被表达（转录）。这些蛋白质通过直接结合DNA中的控制区域来发挥作用。在我们的研究中，我们特别感兴趣的是一种名为Fra2的蛋白质，它作为转录调节因子发挥这种功能。 迄今为止，我们的研究表明，Fra2可能是控制骨骼肌形成的转录因子网络的重要组成部分。因此，我们的研究旨在了解Fra2如何调节与其他因子相关的转录，以及Fra2在基因组中特异性靶向哪些基因。因此，我们将使用最先进的方法在基因组分析中阐明Fra2蛋白在肌肉细胞中的功能。