High performance centrifuges with rotors

带转子的高性能离心机

• 批准号: 422039-2012
• 负责人: Vederas, John
• 金额: $ 8.58万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=495711
• 关键词: performance; centrifuges; rotors

This application requests funding for 3 high performance centrifuges for the preparation of biological samples and the accompanying rotors needed to process a wide range of sample volumes. Typical uses include collecting cells from culture, fractionating cell lysates, purification of DNA and proteins, phase separation of organic mixtures and collection of nano-particles. One centrifuge will be located in a biohazard containment level 2 dedicated room, one centrifuge will be located in the Biological Services facility (a facility providing department-wide access to biology-related equipment), and one centrifuge will be located in the Vederas lab. The rotors and adaptors requested allow for processing of samples several millilitres to litres in size. These centrifuges are used many times per day by approximately 80 members belonging to 15 research groups. It is essential that at least 3 centrifuges be available due to the high number of users spread in location over a 2-wing, 5-story building and also the need to dedicate one centrifuge for biohazard containment level 2 work.

本申请要求资助3台用于制备生物样品的高性能离心机和处理大量样品所需的随附转子。典型的用途包括从培养中收集细胞，分离细胞裂解物，纯化DNA和蛋白质，有机混合物的相分离和纳米颗粒的收集。一台离心机将设在生物危害控制2级专用室，一台离心机将设在生物服务设施（提供全部门使用生物相关设备的设施），一台离心机将设在Vederas实验室。所要求的转子和适配器允许处理几毫升到升大小的样品。这些离心机每天被15个研究小组的大约80名成员多次使用。由于使用者人数众多，分布在一座两翼、五层楼的建筑物内，而且需要一台离心机专门用于2级生物危害控制工作，因此至少需要3台离心机。