Protein and Peptide Chromatographic Purification System with Automated Detection and Collection

具有自动检测和收集功能的蛋白质和肽色谱纯化系统

• 批准号: RTI-2021-00026
• 负责人: Vederas, John
• 金额: $ 9.51万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=718630
• 关键词: Protein; Peptide; Chromatographic; Purification; System

This application requests funding for an automated high pressure system to purify (separate) proteins and peptides (polymers of amino acids). The instrument has computer controlled solvent gradient delivery, multichannel ultraviolet (UV) detection and conductivity detection, and fraction collection. The instrumentation is designed to be compatible with protein solutions (e.g. aqueous buffers under both acid and base conditions) and is able to handle samples with continuous multi-hour operationin a cold room (4 C). The requested equipment can allow isolation of pure proteins and peptides using a wide variety of chromatography columns. These include, but are not limited to, ion exchange, hydrophobic interaction, size exclusion and ligand affinity columns. There is no equivalent instrument with the requested capabilities in the Chemistry Department. The main users will be the 11 Ph.D. students and 3 postdoctoral researchers in the principal applicant's group. However, the equipment will be accessible to all research groups in our department (>12 groups can be expected to frequently use the proposed equipment). It will also be used periodically by other researchers (often collaborators) at the University of Alberta in the Faculties of Science, Medicine, Engineering and Agricultural Life & Environmental Sciences. Proteins (e.g. enzymes) and peptides are at the core of our research effort. Many are drug candidates or are targets of such compounds

该申请要求资助一个自动高压系统，以纯化（分离）蛋白质和肽（氨基酸的聚合物）。该仪器具有计算机控制的溶剂梯度输送、多通道紫外（UV）检测和电导检测以及馏分收集。仪器设计为与蛋白质溶液（例如，酸性和碱性条件下的水性缓冲液）兼容，并且能够在冷室（4 ℃）中连续操作数小时处理样品。所要求的设备可以允许使用各种色谱柱分离纯蛋白质和肽。这些包括但不限于离子交换、疏水相互作用、尺寸排阻和配体亲和柱。化学部没有具有所要求功能的等效仪器。主要用户将是11位博士。主申请人团队中的学生和3名博士后研究人员。然而，该设备将提供给我们部门的所有研究小组（>12组可以预期经常使用拟议的设备）。阿尔伯塔大学的科学、医学、工程和农业生命与环境科学学院的其他研究人员（通常是合作者）也将定期使用它。蛋白质（例如酶）和肽是我们研究工作的核心。许多是候选药物或这些化合物的靶点