Automated Peptide Synthesizer with Integrated Flash Purification System

具有集成快速纯化系统的自动肽合成仪

• 批准号: 472780-2015
• 负责人: Vederas, John
• 金额: $ 10.93万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=564841
• 关键词: Automated; Peptide; Synthesizer; Integrated; Flash

This application requests funding for an automated peptide synthesizer with an integrated purification system as a departmental facility that will also be available to other researchers on campus, especially in the Faculty of Agriculture, Life & Environmental Sciences (ALES). The instrument will be placed in the Biological Services Facility of the Chemistry Department. It will be accessible to all researchers in our department (30 faculty, 213 graduate students: the main users are expected to be the research groups of 7 faculty in the Chemical Biology section). It will also be accessible to the group of Dr. Michael Gaenzle and his collaborators in ALES. Peptides, as short polymers of amino acids, are present in all life forms and have a wide variety of important functions ranging from hormonal signaling to antimicrobial activity. They can be made by well-established methods using automated sequential addition of reagents, including the individual constituent amino acids, to an appropriately activated insoluble solid support. At the end of the multi-day cycle, the finished peptide is cleaved from the solid support and automatically purified. Although manual chemical synthesis of individual peptides is possible and is currently done in our laboratory, the requested instrumentation allows simultaneous independent synthesis of up to 48 different peptides. This greatly increases throughput and efficiency (the instrument can run unattended 24 hours per day), not to mention providing relief from tedious and repetitive procedures for graduate student and postdoctoral researchers.

该申请要求为自动化肽合成仪提供资金，该合成仪具有集成的纯化系统作为部门设施，该设施也将提供给校园内的其他研究人员，特别是农业，生命与环境科学学院（ALES）。仪器将放置在化学部的生物服务机构。它将可供我们部门的所有研究人员使用（30名教师，213名研究生：主要用户预计将是化学生物学部分的7名教师的研究小组）。Michael Gaenzle博士及其在ALES的合作者也可以访问。 肽作为氨基酸的短聚合物，存在于所有生命形式中，并且具有从激素信号传导到抗微生物活性的各种重要功能。它们可以通过成熟的方法制备，使用自动顺序添加试剂，包括单独的组成氨基酸，到适当活化的不溶性固体支持物。在多日循环结束时，将成品肽从固体支持物上切割并自动纯化。虽然单个肽的手动化学合成是可能的，并且目前在我们的实验室中进行，但所要求的仪器允许同时独立合成多达48种不同的肽。这大大提高了通量和效率（仪器可以每天24小时无人值守运行），更不用说为研究生和博士后研究人员提供繁琐和重复的程序。