"Q-PCR system, small volume UV-VIS spectrophotometer and transilluminator for molecular and chemical analyses of food, biomaterials, water and microorganisms"

我们正在申请资金来购买设备，使我们能够(a)分析少量的核酸、蛋白质、抗生素、细胞、脂质及其氧化产物（小体积紫外可见分光光度计），(b)使用基于核酸的基因表达和微生物存在的定量测量（定量聚合酶链反应[Q-PCR]系统）和(c)可视化。记录和操作含有核酸样品的琼脂糖凝胶（透光器），用于身份或基因工程的下游分析。我们的设备需求是长期公共计划的一部分，以建立我们部门的研究能力。收购这些设备对于我们在食品安全、微生物来源追踪、工业新生物的生物挖掘、营养药品开发和增值生化产品生产工艺等领域开展基础和应用项目的能力至关重要。设备使用的例子包括在食品加工过程中分析食源性细菌病原体单核细胞增生李斯特菌的基因表达，定量测定宿主特异性粪便指示细菌（拟杆菌属）和水样中的线粒体DNA标记，海洋和原生生物来源的多不饱和油的氧化质量，委内瑞拉链霉菌生产抗生素（jadomycin）的研究，以及产-3脂肪酸的Thraustochytrids原生生物的鉴定和基因表达

We are requesting funding to purchase equipment that enables us to (a) analyse small quantities of nucleic acids, proteins, antibiotics, cells, lipids and their oxidation products (small volume UV-VIS spectrophotometer), (b) use quantitative nucleic acid based measurements of gene expression and microbial presence (quantitative Polymerase Chain Reaction [Q-PCR] system) and (c) visualise, document and manipulate agarose gels containing samples of nucleic acids (transilluminator) for downstream analysis of identity or genetic engineering. Our equipment request forms part of a long term communal plan to build the research capacity in our department. Acquisition of the equipment is essential for our ability to carry out fundamental and applied projects in the area of food safety, microbial source tracking, biomining for novel organisms of industrial interest, development of nutraceuticals and processes for production of value-added biochemicals. Examples, of equipment uses include analyses of gene expression in the foodborne bacterial pathogen, Listeria monocytogenes, during food processing, quantification of host-specific faecal indicator bacteria (Bacteroidales) and mitochondrial DNA markers in water samples, oxidative quality of polyunsaturated oils of marine and protist origin, antibiotic (jadomycin) production by the Streptomyces venezuelae bacteria and identity of and gene expression in omega-3-fatty acid producing protists belonging to the Thraustochytrids.