"Q-PCR system, small volume UV-VIS spectrophotometer and transilluminator for molecular and chemical analyses of food, biomaterials, water and microorganisms"

我们正在申请资金购买设备，使我们能够(A)分析少量核酸、蛋白质、抗生素、细胞、脂质及其氧化产物(小容量UV-Vis分光光度计)，(B)使用基于核酸的基因表达和微生物存在的定量测量(定量聚合酶链式反应[Q-PCR]系统)，以及(C)可视化、记录和操作含有核酸样本的琼脂糖凝胶(透光仪)，用于下游的身份分析或基因工程。我们的设备请求是建立我们系研究能力的长期公共计划的一部分。购买这些设备对于我们在食品安全、微生物来源追踪、具有工业价值的新生物的生物挖掘、营养食品的开发和增值生物化学品的生产过程中开展基础和应用项目的能力至关重要。设备用途的例子包括在食品加工过程中分析食源性致病菌单核细胞增生性李斯特氏菌的基因表达、对宿主特有的粪便指示菌(类杆菌)和水样中线粒体DNA标记的量化、海洋和原生生物来源的多不饱和油脂的氧化质量、维尼链霉菌生产的抗生素(Jadmycin)以及属于甲藻的omega-3-脂肪酸产生原生生物的特性和基因表达。

We are requesting funding to purchase equipment that enables us to (a) analyse small quantities of nucleic acids, proteins, antibiotics, cells, lipids and their oxidation products (small volume UV-VIS spectrophotometer), (b) use quantitative nucleic acid based measurements of gene expression and microbial presence (quantitative Polymerase Chain Reaction [Q-PCR] system) and (c) visualise, document and manipulate agarose gels containing samples of nucleic acids (transilluminator) for downstream analysis of identity or genetic engineering. Our equipment request forms part of a long term communal plan to build the research capacity in our department. Acquisition of the equipment is essential for our ability to carry out fundamental and applied projects in the area of food safety, microbial source tracking, biomining for novel organisms of industrial interest, development of nutraceuticals and processes for production of value-added biochemicals. Examples, of equipment uses include analyses of gene expression in the foodborne bacterial pathogen, Listeria monocytogenes, during food processing, quantification of host-specific faecal indicator bacteria (Bacteroidales) and mitochondrial DNA markers in water samples, oxidative quality of polyunsaturated oils of marine and protist origin, antibiotic (jadomycin) production by the Streptomyces venezuelae bacteria and identity of and gene expression in omega-3-fatty acid producing protists belonging to the Thraustochytrids.