Immune responses to staphylococcus aureus

对金黄色葡萄球菌的免疫反应

• 批准号: 3525-2008
• 负责人: Talbot, Brian
• 金额: $ 2.5万
• 依托单位: Université de Sherbrooke
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=498106
• 关键词: Immune; responses; staphylococcus; aureus

Staphylococcus aureus is a common bacteria which, in its antibiotic resistant form (MRSA), has become a common and dangerous hospital infection. It also causes mastitis in dairy cattle which is responsible for serious losses to the dairy industry. Unfortunately, most vaccines described up until now are only partially protective and are specific to certain types of infection. This research program which has been underway for several years was designed to study how S. aureus causes changes in the protective responses (immune responses) of infected animals and how these responses alter the efficiency of vaccines. A combination of vaccines made from genetic material (DNA) and proteins of the bacterium has been injected into mice which were then infected with the bacteria. Such DNA/protein vaccines are a relatively new technology that has recently been licensed for some fish viruses and West Nile virus in horses. Our results have suggested that each manifestation of S. aureus infection, be it arthritis, septicemia, endocarditis or mastitis, stimulates a slightly different immune response by the animal. Vaccination against the infection in one part of the body does not necessarily protect against infections at other sites. Thus it is important to identify how the immune responses differ from infection to infection. The immediate objective of this program is to study how the immune responses to S. aureus in mouse models vary during infections at different sites. The longer-term objectives of this program will be to find out how the immune response changes after the animals have been injected with different prototype vaccines. We will continue to use mouse models of S. aureus-induced septicemia, arthritis and mastitis, but we will also use a simulation of the bacterial colonization of the nose. This is often where the bacteria appears to be present in humans but without causing any symptoms. This "nasal carriage" may explain how antibiotic resistant bacteria have spread so fast. The success of such a project will have an important impact in the fight against other emerging and potentially dangerous bacteria.

金黄色葡萄球菌（Staphylococcus aureus）是一种常见的细菌，其耐抗生素形式（MRSA）已成为常见且危险的医院感染。它还导致奶牛乳房炎，这对乳制品行业造成严重损失。不幸的是，迄今为止描述的大多数疫苗仅具有部分保护性，并且仅针对某些类型的感染。本研究计划已进行了数年，旨在研究S。金黄色葡萄球菌会导致感染动物的保护性反应（免疫反应）发生变化，以及这些反应如何改变疫苗的效率。 一种由细菌的遗传物质（DNA）和蛋白质制成的疫苗组合被注射到小鼠体内，然后感染细菌。这种DNA/蛋白质疫苗是一种相对较新的技术，最近已被许可用于一些鱼类病毒和马的西尼罗河病毒。我们的结果表明，S。金黄色葡萄球菌感染，无论是关节炎、败血症、心内膜炎还是乳腺炎，都会刺激动物产生略微不同的免疫应答。针对身体某个部位的感染接种疫苗并不一定能保护其他部位免受感染。因此，重要的是要确定免疫反应如何从感染到感染。本项目的近期目标是研究免疫系统对S.小鼠模型中的金黄色葡萄球菌在不同部位感染期间变化。该计划的长期目标是找出动物注射不同原型疫苗后免疫反应的变化。我们将继续使用S的小鼠模型。金黄色葡萄球菌引起的败血症，关节炎和乳腺炎，但我们也将使用模拟鼻子的细菌定植。这通常是细菌似乎存在于人体中，但不会引起任何症状的地方。 这种“鼻腔运输”可能解释了抗生素耐药细菌如何传播得如此之快。这一项目的成功将对对抗其他新出现的潜在危险细菌产生重要影响。