Cellular mechanisms implicated in the regulation of the group IVA phospholipase A2 and lipid mediators biosynthesis in activited cells

参与调节活化细胞中 IVA 族磷脂酶 A2 和脂质介质生物合成的细胞机制

• 批准号: 355670-2009
• 负责人: Flamand, Nicolas
• 金额: $ 2.7万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=498145
• 关键词: Cellular; mechanisms; implicated; regulation; group

Our research group focuses at defining the molecular mechanisms implicated in the regulation of the cPLA2alpha, a key protein involved in the formation of several bioactive lipids. More precisely, the cPLA2alpha releases important components of cell membranes that are further transformed into potent bioactive lipids. Too much of these lipids worsens inflammatory diseases like arthritis and asthma. Therefore, understanding how our body generates these lipids is very important in order to prevent their bad effect. In this proposal, we aim at understanding the mechanisms by which cyclic AMP prevents the activation of the cPLA2alpha in activated cells. Our experimental approach will allow us to decipher all the steps implicated in this effect of cyclic AMP both in vitro and in human cells. This work will not only provide key notions about how the activity of the cPLA2alpha is controlled in our body. It will also serve to train undergraduate and graduate students to become highly qualified scientists.

我们的研究小组专注于确定与cpla2 α调控有关的分子机制，cpla2 α是一种参与几种生物活性脂质形成的关键蛋白。更准确地说，cPLA2alpha释放细胞膜的重要成分，这些成分进一步转化为有效的生物活性脂质。过多的脂质会加重关节炎和哮喘等炎症性疾病。因此，了解我们的身体如何产生这些脂质是非常重要的，以防止它们的不良影响。在本研究中，我们旨在了解环AMP在活化细胞中阻止cpla2 α活化的机制。我们的实验方法将使我们能够在体外和人类细胞中破译与环AMP的这种作用有关的所有步骤。这项工作不仅将提供关于如何在我们体内控制cPLA2alpha活性的关键概念。它还将有助于培养本科生和研究生成为高素质的科学家。