A genetic approach to discover novel regulators of microtubule behaviour

发现微管行为新调节因子的遗传方法

• 批准号: 341474-2007
• 负责人: Srayko, Martin
• 金额: $ 2.63万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=498187
• 关键词: genetic; approach; discover; novel; regulators

The goal of our research is to understand how microscopic structures such as the mitotic spindle assemble in living cells. Like most complex 4-dimensional biological processes, a complete understanding of spindle assembly will depend on knowledge of the properties of individual components as well as an appreciation for how their assembly is orchestrated in living cells. We use the nematode Caenorhabditis elegans to study this problem because microtubule structures are assembled and disassembled rapidly in the first cell division, and many regulatory molecules are likely required to provide the instructions. The importance of microtubules to cell division, and their sensitivity to such molecular instruction, is perhaps best evidenced by the fact that many anti-cancer drugs target the microtubule polymer (e.g. Taxol and Vinblastine). However, we do not have a complete understanding of how microtubule growth and disassembly is regulated within the cell.

我们研究的目标是了解有丝分裂纺锤体等微观结构如何在活细胞中组装。 像大多数复杂的4维生物过程一样，对纺锤体组装的完整理解将取决于对单个组件特性的了解，以及对它们在活细胞中如何组装的理解。 我们使用线虫秀丽隐杆线虫来研究这个问题，因为微管结构在第一次细胞分裂中迅速组装和分解，并且可能需要许多调节分子来提供指令。 微管对细胞分裂的重要性，以及它们对这种分子指令的敏感性，可能最好地证明了许多抗癌药物靶向微管聚合物（例如紫杉醇和长春碱）的事实。 然而，我们没有一个完整的了解微管的生长和拆卸是如何在细胞内调节。