Biological importance of C-3 alpha epimer of 25-hydroxy vitamin D regarding bone mineral density in young and adult rats

25-羟基维生素 D 的 C-3 α 差向异构体对幼年和成年大鼠骨矿物质密度的生物学重要性

• 批准号: 424369-2011
• 负责人: Weiler, Hope
• 金额: $ 3.65万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=498596
• 关键词: Biological; importance; alpha; epimer; 25

Vitamin D is a nutrient that helps bones grow and stay strong. In Canada, the main dietary source of vitamin D comes from milk. Milk has vitamin D added in amounts that help to prevent the body from having very low stores of this vitamin D. Recently, a new form of vitamin D, called C-3 epimer, has been discovered in blood samples taken from infants and adults. The only difference is that one chemical bond has a different orientation, however, this might alter how well it works. For example, vitamin D works by binding to receptors in the body's cells in a similar way as using a key in a lock to open a door. A small change could alter how the key works. Therefore, it is unclear if the C-3 epimer is as good as the normal form of vitamin D in helping bones grow and stay strong. This research is designed to test if higher intakes of vitamin D result in proportional increases in C-3 epimer and to test if this has any consequences to growing or adult bone. The work will be conducted in young and mature rats over 8 weeks of feeding various amounts of the vitamin D forms. Bone will be examined by taking a series of x-rays specially designed to show how much calcium is packed into bone and also to estimate bone strength. The results will help the dairy industry to know where the C-3 epimer comes from and if it is good for bone.

维生素D是一种帮助骨骼生长和保持强壮的营养素。 在加拿大，维生素D的主要饮食来源来自牛奶。 牛奶中添加了大量的维生素D，有助于防止身体缺乏这种维生素D。 最近，在婴儿和成人的血液样本中发现了一种新形式的维生素D，称为C-3差向异构体。 唯一的区别是一个化学键有不同的方向，然而，这可能会改变它的工作方式。 例如，维生素D通过与人体细胞中的受体结合来发挥作用，其方式与使用锁中的钥匙打开门的方式类似。一个小小的改变就能改变钥匙的工作方式。 因此，目前还不清楚C-3差向异构体是否与正常形式的维生素D一样有助于骨骼生长和保持强壮。这项研究旨在测试维生素D的摄入量增加是否会导致C-3差向异构体的比例增加，并测试这是否对生长或成人骨骼有任何影响。 这项工作将在年轻和成熟的大鼠中进行，为期8周，喂食不同数量的维生素D形式。 骨骼将通过一系列专门设计的X光片进行检查，以显示有多少钙填充到骨骼中，并估计骨骼强度。 研究结果将有助于乳品行业了解C-3差向异构体的来源以及它是否对骨骼有益。