Discovery, characterization and applications testing of fungal enzymes for white biotechnology

作为全球最丰富的可再生生物质来源，植物衍生的木质纤维素是用于生产生物燃料和化学品的有吸引力的原料。预处理原料的酶水解是目前将木质纤维素生物质中存在的大聚合物转化为用于化学品和生物燃料生产的小分子的最有效方法。该提案的目标是鉴定酶和辅助蛋白，其增强预处理的木质纤维素原料酶促转化为化学品和生物燃料生产所需的单糖。我们还对应用于纸浆和造纸、纺织、粮食和饲料工业的木质纤维素活性酶和辅助蛋白感兴趣。我们发现在这些领域应用的酶的方法将是克隆，生物化学表征和对我们的工业合作伙伴Iogen Inc.感兴趣的领域中具有潜在商业应用的蛋白质进行工业相关应用测试。Iogen的商业利益领域包括：生物燃料、纸浆和纸张、纺织品、谷物和饲料。新的酶和辅助蛋白将使用尼日尔曲霉或巴斯德毕赤酵母表达为分泌蛋白。

Being the most abundant global source of renewable biomass, plant derived lignocellulose is an attractive feedstock for the production of biofuels and chemicals. Enzymatic hydrolysis of pretreated feedstocks is presently the most effective method for converting the large polymers present in lignocellulose biomass into the small molecules used for chemicals and biofuels production. A goal of this proposal is to identify enzymes and accessory proteins that enhance the enzymatic conversion of pretreated lignocellulose feedstocks into the simple sugars needed for the production of chemicals and biofuels. We are also interested in lignocellulose active enzymes and accessory proteins that have applications in the pulp and paper, textiles, grain and feed industries. Our approach for discovering enzymes with applications in these areas will be to clone, biochemically characterize and perform industrially relevant applications testing on proteins that have potential commercial application in areas of interest to our industrial partner, Iogen Inc. Iogen's areas of commercial interest are; biofuels, pulp and paper, textiles, grain and feed. New enzymes and accessory proteins will be expressed as secreted proteins using either Aspergillus niger or Pichia pastoris.