Zebrafish housing unit and biosafety cabinet

斑马鱼饲养单元和生物安全柜

• 批准号: 440442-2013
• 负责人: Peng, Chun
• 金额: $ 2.23万
• 依托单位: York University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=506197
• 关键词: Zebrafish; housing; unit; biosafety; cabinet

Zebrafish is an excellent model organism to study developmental and physiological processes and to investigate events related to human diseases. We have been using zebrafish to explore how hormones and growth factors regulate reproduction, particularly egg development and maturation. We would like to use zebrafish as a model to study how ovarian cancer cells invade other tissues and organs and to examine the functions of genes in blood vessel formation. In this proposal, funding is requested to purchase a stand-alone zebrafish housing rack system and a biosafety cabinet. The housing system is equipped with filters and ultra-violet sterilizers, as well as control and monitoring system to ensure optimal water quality. This provides a healthy and comfortable environment for zebrafish. A biosafety cabinet is required to protect researchers from potential biohazardous agents when perform experimental procedure and to keep the cell and tissue cultures free from potential contaminations. These instruments will allow us to conduct innovative research that will not only enhance our understanding on how reproductive functions are controlled, but also have potential clinical applications.

斑马鱼是研究发育和生理过程以及研究与人类疾病相关事件的优秀模式生物。 我们一直在利用斑马鱼来探索激素和生长因子如何调节繁殖，特别是卵子的发育和成熟。 我们想用斑马鱼作为模型来研究卵巢癌细胞如何侵入其他组织和器官，并检查基因在血管形成中的功能。 在该提案中，请求资金购买独立的斑马鱼饲养架系统和生物安全柜。 外壳系统配备过滤器和紫外线消毒器，以及控制和监测系统，以确保最佳的水质。 这为斑马鱼提供了一个健康舒适的环境。 需要生物安全柜来保护研究人员在进行实验过程时免受潜在生物危害剂的影响，并保持细胞和组织培养物免受潜在污染。 这些仪器将使我们能够进行创新研究，不仅可以增强我们对生殖功能如何控制的理解，而且具有潜在的临床应用。