Endocrine control of fish reproduction

鱼类繁殖的内分泌控制

• 批准号: 203081-2009
• 负责人: Peng, Chun
• 金额: $ 4.98万
• 依托单位: York University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=514075
• 关键词: Endocrine; control; fish; reproduction

Animal reproductive functions require precise hormonal control. Members of the transforming growth factor-beta (TGF-beta) family have been found to play important roles in regulating ovarian functions; however, their precise physiological roles are not fully understood. We have been studying the structure and function of several members of the TGF-beta family and demonstrated that activin stimulates, while TGF-beta and bone morphogenetic protein (BMP)-15 inhibit, maturation of eggs in the zebrafish ovary. In the next five years, we will continue to use zebrafish as a model to address the following questions: 1) what are the physiological significance of activin, TGF-beta and BMP-15 in egg maturation and ovulation? 2) how do these factors regulate egg maturation? 3) what modulates the expression and activities of these factors? We will first use gain- and loss-of-functions approaches to determine the physiological roles of activin, TGF-beta, and BMP-15. We will test if these factors regulate egg maturation mainly by regulating the responsiveness of eggs to maturation-inducing hormones. Finally, we will examine how the production and activity of these factors are controlled by other hormones and by a group of small RNAs, known as microRNA. The proposed studies will provide novel insight into the regulation, actions, and signaling pathways of the TGF-beta family in the ovary. They will enhance our overall understanding of vertebrate reproduction. Finally, these studies may also have potential application into aquaculture.

动物的生殖功能需要精确的激素控制。转化生长因子- β (tgf - β)家族成员已被发现在调节卵巢功能中发挥重要作用；然而，它们的确切生理作用尚不完全清楚。我们一直在研究tgf - β家族的几个成员的结构和功能，并证明激活素刺激斑马鱼卵巢中卵子的成熟，而tgf - β和骨形态发生蛋白(BMP)-15抑制卵子的成熟。在未来五年内，我们将继续以斑马鱼为模型，解决以下问题：1)激活素、tgf - β和BMP-15在卵子成熟和排卵中的生理意义是什么？2)这些因素是如何调控卵子成熟的？3)是什么调节了这些因子的表达和活动？我们将首先使用功能增益和功能丧失的方法来确定激活素、tgf - β和BMP-15的生理作用。我们将测试这些因素是否主要通过调节卵子对诱导成熟激素的反应来调节卵子成熟。最后，我们将研究这些因子的产生和活性如何受到其他激素和一组小rna（称为microRNA）的控制。提出的研究将为卵巢中tgf - β家族的调控、作用和信号通路提供新的见解。它们将增强我们对脊椎动物繁殖的全面了解。最后，这些研究也可能在水产养殖中有潜在的应用。