(Ultra)microtome for thin and ultrathin sectioning needed for light and electron microscope study of dwarf mistletoe anatomy and reproductive development.

用于矮槲寄生解剖学和生殖发育的光学和电子显微镜研究所需的薄和超薄切片的(超)切片机。

基本信息

  • 批准号:
    439895-2013
  • 负责人:
  • 金额:
    $ 5.33万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Research Tools and Instruments - Category 1 (<$150,000)
  • 财政年份:
    2012
  • 资助国家:
    加拿大
  • 起止时间:
    2012-01-01 至 2013-12-31
  • 项目状态:
    已结题

项目摘要

My NSERC-funded program is dedicated to investigating reproductive development in dwarf mistletoes (genus Arceuthobium), parasitic flowering plants that infect economically important conifers. Techniques of microscopy are the foundation of the work my students and I do. Presently, we are investigating developmental and dispersive events of Arceuthobium americanum (lodgepole pine dwarf mistletoe) in British Columbia. As effective maturation of pollen and its dissemination govern the spread of Arceuthobium as a parasite, our work will identify key stages toward which a control program may ultimately be devised. We aim to use light microscopy (LM), fluorescence microscopy (FM), and transmission electron microscopy (TEM) to examine developmental anatomy and wall chemistry of anthers and pollen. The only way this can be accomplished is to use a versatile microtome/ultramicrotome to prepare thin sections for LM and FM, and ultrathin sections for TEM. Students are drawn to work with me because of the excellent skills in microscopy and microtomy acquired. When I first arrived at Thompson Rivers University in 2004, I inherited a Sorvall MT-2 ultramicrotome circa 1965. This is the sole instrument my students and I have been using to obtain sections for our exciting research results published to date in good journals (e.g., Annals of Botany). I had been able to limp the Sorvall along by having it serviced regularly and performing maintenance on it myself. However, shortly after my first NSERC DG renewal in 2012, the Sorvall became unusable for all but the crudest sectioning and basic training. There is no other ultramicrotome within 200 km. To make use of the DG, we desperately need a new, modern accessorized (ultra)microtome capable of thin and ultrathin sectioning. My program threatens to stall because of the Sorvall's 'passing'. Through my NSERC DG, I proposed to train 13 undergraduate and 3 graduate students as well as contribute information that can be used to shape management decisions regarding this notorious forest pest. Such outcomes, beneficial to Canada through generation of new researchers and new information for foresters, can only be made possible through the acquisition of an (ultra)microtome.
我的NSERC资助的项目致力于研究矮生槲寄生(Arceuthobium属)的生殖发育,这种寄生开花植物会感染经济上重要的针叶树。显微镜技术是我和我的学生所做工作的基础。目前,我们正在调查不列颠哥伦比亚省美洲根瘤菌(Arceuthobium Americanum)的发育和扩散事件。由于花粉的有效成熟及其传播控制着Arceuthobium作为寄生虫的传播,我们的工作将确定最终可能制定控制程序的关键阶段。我们的目标是使用光学显微镜(LM)、荧光显微镜(FM)和透射电子显微镜(TEM)来研究花药和花粉的发育解剖和壁化学。唯一可以做到这一点的方法是使用多功能切片机/超切片机来制备用于光学显微镜和调频的薄片,以及用于透射电子显微镜的超薄切片。学生被吸引到和我一起工作,因为我在显微镜和显微切割术方面获得了出色的技能。2004年,当我第一次来到汤普森·里弗斯大学时,我继承了一台大约在1965年左右的索瓦尔MT-2超微切割机。这是我和我的学生们一直在使用的唯一工具,用来获得我们令人兴奋的研究成果的部分,这些研究成果迄今已在优秀期刊(如《植物学年鉴》)上发表。通过定期维修和自己进行维护,我一直能够让Sorvall一瘸一拐的。然而,在2012年我第一次更新NSERC DG后不久,Sorvall变得无法使用,除了最原始的切片和基本训练。在200公里的范围内没有其他的超微切割机。为了利用DG,我们迫切需要一种新的、现代的配饰(超)切割机,能够进行薄和超薄切片。我的程序可能会因为索瓦尔的“通过”而停滞。通过我的NSERC DG,我提议培训13名本科生和3名研究生,并提供可用于制定针对这种臭名昭著的森林有害生物的管理决策的信息。通过产生新的研究人员和为林农提供新的信息,这样的结果对加拿大有利,只有通过购买(超)显微切割机才能实现。

项目成果

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RossFriedman, Cynthia其他文献

RossFriedman, Cynthia的其他文献

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{{ truncateString('RossFriedman, Cynthia', 18)}}的其他基金

Reproductive development in dwarf mistletoes (genus Arceuthobium): the male's tale, explosive seed discharge, and 'environmental' microscopy.
矮槲寄生(Arceuthobium 属)的生殖发育:雄性的故事、爆炸性种子排放和“环境”显微镜。
  • 批准号:
    327214-2012
  • 财政年份:
    2016
  • 资助金额:
    $ 5.33万
  • 项目类别:
    Discovery Grants Program - Individual
Reproductive development in dwarf mistletoes (genus Arceuthobium): the male's tale, explosive seed discharge, and 'environmental' microscopy.
矮槲寄生(Arceuthobium 属)的生殖发育:雄性的故事、爆炸性种子排放和“环境”显微镜。
  • 批准号:
    327214-2012
  • 财政年份:
    2015
  • 资助金额:
    $ 5.33万
  • 项目类别:
    Discovery Grants Program - Individual
Reproductive development in dwarf mistletoes (genus Arceuthobium): the male's tale, explosive seed discharge, and 'environmental' microscopy.
矮槲寄生(Arceuthobium 属)的生殖发育:雄性的故事、爆炸性种子排放和“环境”显微镜。
  • 批准号:
    327214-2012
  • 财政年份:
    2014
  • 资助金额:
    $ 5.33万
  • 项目类别:
    Discovery Grants Program - Individual
Reproductive development in dwarf mistletoes (genus Arceuthobium): the male's tale, explosive seed discharge, and 'environmental' microscopy.
矮槲寄生(Arceuthobium 属)的生殖发育:雄性的故事、爆炸性种子排放和“环境”显微镜。
  • 批准号:
    327214-2012
  • 财政年份:
    2013
  • 资助金额:
    $ 5.33万
  • 项目类别:
    Discovery Grants Program - Individual
The Canadian Botanical Association Meeting & Conference Returns to the Interior Region of BC after a 12-year Absence!
加拿大植物协会会议
  • 批准号:
    447738-2013
  • 财政年份:
    2013
  • 资助金额:
    $ 5.33万
  • 项目类别:
    Regional Office Discretionary Funds
Reproductive development in dwarf mistletoes (genus Arceuthobium): the male's tale, explosive seed discharge, and 'environmental' microscopy.
矮槲寄生(Arceuthobium 属)的生殖发育:雄性的故事、爆炸性种子排放和“环境”显微镜。
  • 批准号:
    327214-2012
  • 财政年份:
    2012
  • 资助金额:
    $ 5.33万
  • 项目类别:
    Discovery Grants Program - Individual
Viscin tissue of the dwarf misteltoe Arceuthobium americanum (Viscaceae): how does it develop, what is it made of, and how does it function in explosive seed discharge
矮槲寄生 Arceuthobium americanum(Viscaceae)的粘蛋白组织:它是如何发育的,它是由什么组成的,以及它在爆炸性种子排放中如何发挥作用
  • 批准号:
    327214-2006
  • 财政年份:
    2011
  • 资助金额:
    $ 5.33万
  • 项目类别:
    Discovery Grants Program - Individual
Viscin tissue of the dwarf misteltoe Arceuthobium americanum (Viscaceae): how does it develop, what is it made of, and how does it function in explosive seed discharge
矮槲寄生 Arceuthobium americanum(Viscaceae)的粘蛋白组织:它是如何发育的,它是由什么组成的,以及它在爆炸性种子排放中如何发挥作用
  • 批准号:
    327214-2006
  • 财政年份:
    2010
  • 资助金额:
    $ 5.33万
  • 项目类别:
    Discovery Grants Program - Individual

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用于无载玻片和数字组织学的深度学习显微镜
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