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Respirometry and environmental control in micro aquatic systems

微型水生系统中的呼吸测量和环境控制

基本信息

批准号：
439917-2013
负责人：
Wright, Patricia
金额：
$ 5.92万
依托单位：
University of Guelph
依托单位国家：
加拿大
项目类别：
Research Tools and Instruments - Category 1 (<$150,000)
财政年份：
2012
资助国家：
加拿大
起止时间：
2012-01-01 至 2013-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=506226
关键词：
Respirometry environmental control micro aquatic

项目摘要

Animals consume oxygen in order to harvest energy from proteins, carbohydrates and fats obtained in food. The amount of oxygen an animal consumes changes with body size, exercise, stress, temperature, daily body rhythms, among other variables. Accurate measurement of oxygen uptake, a good proxy for metabolic rate provides fundamental information on an animal's physiological status. Oxygen uptake can be measured in aquatic breathers by measuring the amount of oxygen removed from the external water per unit time. In air breathers, the measurement can be complicated by the relatively high oxygen concentrations in air, especially if the organisms are relatively small. We are requesting funds to purchase state-of-the-art respirometry equipment to precisely measure resting oxygen consumption in small aquatic and amphibious fishes at all stages of development from individual embryos (1 mm) to adults (< 3 cm) in water or air. These resting measurements will be followed up by measurements of oxygen uptake in swimming or active fish using specialized micro swim tunnels to assess performance after previous exposure to environmental perturbations. Environmental perturbations include low oxygen (hypoxia) and elevated carbon dioxide levels (hypercapnia) in the environment. We request funds to purchase two independent systems, one for oxygen, the other for carbon dioxide to precisely regulate these parameters in fish tanks for short (eg. < 1 day) or long periods of time (eg. > 1 month). Data is collected and analyzed by a computer interface. The equipment requested is fundamental to four NSERC-funded research programs and will be used weekly.

动物消耗氧气是为了从食物中获得的蛋白质、碳水化合物和脂肪中获取能量。动物消耗的氧气量随着体型、运动、压力、温度、日常身体节律以及其他变量而变化。准确测量摄氧量，是代谢率的一个很好的代表，为动物的生理状态提供了基本信息。通过测量每单位时间从外部水中去除的氧气量，可以测量水生呼吸者的摄氧量。在空气呼吸者中，由于空气中相对较高的氧浓度，特别是在生物相对较小的情况下，测量可能会变得复杂。我们正在申请资金购买最先进的呼吸测量设备，以精确测量小型水生和两栖鱼类在水或空气中从个体胚胎（1毫米）到成鱼（< 3厘米）的所有发育阶段的静息耗氧量。在这些静息测量之后，将使用专门的微游隧道测量游泳或活动鱼类的摄氧量，以评估先前暴露于环境扰动后的表现。环境扰动包括环境中的低氧（缺氧）和二氧化碳水平升高（高碳酸血症）。我们要求资金购买两个独立的系统，一个用于氧气，另一个用于二氧化碳，以精确调节鱼缸中的这些参数。< 1天)或较长时间(如：> 1个月)。数据通过计算机接口收集和分析。所要求的设备是nserc资助的四个研究项目的基础，将每周使用一次。