Effect of metallic nanoparticles on functional and structural properties of the photosynthetic apparatus

光系统II结构和电子传递活性的调节受到环境因素的强烈影响，如不同污染物的存在会造成抑制效应。在这项研究计划中，我们试图了解光合作用装置的结构和功能是如何适应这些环境条件的。光合作用过程对环境胁迫的高度敏感性可用于开发生物分析工具，以评估环境中污染物的毒理风险。由于近年来纳米技术的发展和纳米材料在环境中被发现的可能性，人们开始评估不同类型的纳米粒子对光合作用生物的可能影响，我们的假设是，在这些条件下，能量耗散机制将反映出纳米粒子诱导抑制机制的特殊性。我们的研究目的是研究在强光强下诱导的缓蚀剂和纳米颗粒的作用与能量耗散机制之间的关系。通过使用非常先进的光谱研究和生化方法，将在体外和体内条件下进行实验。所获得的结果将为解释植物如何在不同的光照条件下生存和耐受纳米颗粒的毒性效应提供信息。这些结果将有助于了解一些纳米颗粒的抑制效应与光合作用电子传递之间的相互作用的基本知识，以及评价毒性生物标志物识别纳米颗粒诱导效应的可靠性。

The regulation of photosystem II structure and electron transport activity is strongly affected by environmental factors such as the presence of different pollutants which cause inhibitory effects. In this research program, we attempt to understand in which way structure and functions of photosnthetic apparatus are accommodated to cope with those environmental conditions. The high sensitivity of photosynthetic processes towards environmental stress can be used for the development of bioanalytical tools to evaluate the toxicological risk of contaminants in the environment. Due to the recent development of nanotechnology and the possibility of nanomaterials being found in the environment, an evaluation of the possible effects of different types of nanoparticles on photosynthetic organisms was initiated.Our hypothesis is that, under those conditions, the mechanism of energy dissipation will reflect the specificity of the inhibition mechanism induced by nanoparticles. Our research objective is to study the relationships between the effects of inhibitors, nanoparticles induced under strong light intensity and energy dissipation mechanisms. By using highly advanced spectroscopic studies and biochemical methods, experiments will be done in vitro and in vivo conditions. Results obtained will provide information which can explain how plants can survive and tolerate nanoparticles toxicity effects under different light conditions. These results will contribute to fundamental knowledge concerning the interaction between inhibitory effects of some nanoparticles and on photosynthetic electron transport the necessity and to evaluate the reliability of toxicity biomarkers to identify nanoparticles-induced effects.