Designer Peptides and Proteins: Characterization by High Pressure Liquid Chromatography

设计肽和蛋白质：高压液相色谱表征

• 批准号: 440140-2013
• 负责人: Shoichet, Molly
• 金额: $ 5.1万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=509720
• 关键词: Designer; Peptides; Proteins; Characterization; Pressure

Key to chemical biology is purity of materials, whether for the design of new peptide/protein-modified hydrogels, drug colloids, or metal-chelating polymers. The high pressure liquid chromatograph (HPLC) allows both biomolecules to be purified and polymerization reaction kinetics to be quantified. The HPLC is critical to success in the advanced research being pursued by the laboratories of Molly Shoichet, Mitchell Winnik and Brian Shoichet. In the combined laboratories, there are 70 highly qualified personnel who will benefit directly from the HPLC, plus another 100+ researchers in our collaborators' laboratories. Importantly, the proposed HPLC has become a mainstay of the modern laboratory as reflected in the multi-disciplinary research proposed by principal investigators from three distinct faculties: Applied Science & Engineering (MS Shoichet), Arts & Science (MA Winnik) and Pharmaceutical Sciences (BK Shoichet). With limited access to other HPLC instruments on campus and the near standstill to which the laboratory was taken when an old HPLC was non-functional this past summer, it is clear that the HPLC has a significant impact on the pace of research. The information obtained from the HPLC will allow us to advance our knowledge-based economy, particularly in the fields of immunoassays, cell delivery and drug formulations. This is clear from our multiple points of contact and collaboration with industry in Canada and worldwide. Commercialization of MA Winnik's metal-chelating polymers has been demonstrated with an Ontario-based company; CCRM, in collaboration with a large pharmaceutical company, has an option to license one of MS Shoichet's inventions and is interested in having access to this HPLC, thereby further strengthening our ties to Canadian industry.

化学生物学的关键是材料的纯度，无论是用于设计新的肽/蛋白质修饰的水凝胶，药物胶体还是金属螯合聚合物。 高压液相色谱（HPLC）允许纯化生物分子和定量聚合反应动力学。 HPLC是Molly Shoichet、Mitchell Winnik和Brian Shoichet实验室进行的高级研究取得成功的关键。 在合并后的实验室中，有70名高素质的人员将直接受益于HPLC，另外还有100多名合作实验室的研究人员。 重要的是，拟议的HPLC已成为现代实验室的支柱，这反映在来自三个不同学院的主要研究人员提出的多学科研究中：应用科学与工程（MS Shoichet），艺术与科学（MA Winnik）和药物科学（BK Shoichet）。 由于校园内使用其他HPLC仪器的机会有限，并且在去年夏天旧HPLC不起作用时实验室几乎处于停顿状态，很明显HPLC对研究的步伐有着重大影响。 从HPLC获得的信息将使我们能够推进我们的知识经济，特别是在免疫测定，细胞递送和药物制剂领域。 这一点从我们与加拿大和全球工业界的多个接触点和合作中可以清楚地看出。MA Winnik的金属螯合聚合物的商业化已经与安大略省的一家公司进行了论证; CCRM与一家大型制药公司合作，可以选择许可MS Shoichet的一项发明，并有兴趣使用这种HPLC，从而进一步加强我们与加拿大工业的联系。