Designer Peptides and Proteins: Characterization by High Pressure Liquid Chromatography

化学生物学的关键是材料的纯度，无论是设计新的肽/蛋白质修饰的水凝胶，药物胶体还是金属螯合聚合物。高压液相色谱仪（HPLC）既可以纯化生物分子，又可以量化聚合反应动力学。高效液相色谱法是莫莉·休伊切特、米切尔·温尼克和布莱恩·休伊切特实验室所追求的先进研究成功的关键。在合并的实验室中，有70名高素质的人员将直接受益于HPLC，另外还有100多名研究人员在我们的合作实验室中。重要的是，提议的高效液相色谱已成为现代实验室的支柱，这反映在三个不同院系的主要研究人员提出的多学科研究：应用科学与工程（MS Shoichet），艺术与科学（MA Winnik）和制药科学（BK Shoichet）。由于校园内其他高效液相色谱仪器的使用受限，而且由于去年夏天一台旧的高效液相色谱仪无法正常工作，实验室几乎处于停滞状态，因此很明显，高效液相色谱仪对研究速度产生了重大影响。从HPLC中获得的信息将使我们能够推进我们的知识型经济，特别是在免疫分析、细胞传递和药物配方领域。从我们与加拿大和世界各地的行业的多个接触和合作中可以清楚地看出这一点。MA Winnik的金属螯合聚合物的商业化已经与安大略省的一家公司进行了演示；CCRM与一家大型制药公司合作，拥有Shoichet的一项发明的授权，并有兴趣获得这种高效液相色谱，从而进一步加强我们与加拿大工业界的联系。

Key to chemical biology is purity of materials, whether for the design of new peptide/protein-modified hydrogels, drug colloids, or metal-chelating polymers. The high pressure liquid chromatograph (HPLC) allows both biomolecules to be purified and polymerization reaction kinetics to be quantified. The HPLC is critical to success in the advanced research being pursued by the laboratories of Molly Shoichet, Mitchell Winnik and Brian Shoichet. In the combined laboratories, there are 70 highly qualified personnel who will benefit directly from the HPLC, plus another 100+ researchers in our collaborators' laboratories. Importantly, the proposed HPLC has become a mainstay of the modern laboratory as reflected in the multi-disciplinary research proposed by principal investigators from three distinct faculties: Applied Science & Engineering (MS Shoichet), Arts & Science (MA Winnik) and Pharmaceutical Sciences (BK Shoichet). With limited access to other HPLC instruments on campus and the near standstill to which the laboratory was taken when an old HPLC was non-functional this past summer, it is clear that the HPLC has a significant impact on the pace of research. The information obtained from the HPLC will allow us to advance our knowledge-based economy, particularly in the fields of immunoassays, cell delivery and drug formulations. This is clear from our multiple points of contact and collaboration with industry in Canada and worldwide. Commercialization of MA Winnik's metal-chelating polymers has been demonstrated with an Ontario-based company; CCRM, in collaboration with a large pharmaceutical company, has an option to license one of MS Shoichet's inventions and is interested in having access to this HPLC, thereby further strengthening our ties to Canadian industry.