Ultra centrifuge for extraction of high value biomolecules

所要求的Micro-Ultra离心机在造粒亚细胞颗粒、病毒、DNA和RNA、蛋白质纯化和分离、脂蛋白的差异浮选、细胞细胞器(核糖体、线粒体、微粒体)和纳米颗粒的分离方面非常有效。该部门对加利博士、布鲁克斯博士、巴奇博士、加尼姆博士、吉尔博士和耶尔迪兹博士的研究小组非常重要。目前的装置已有46年的历史，已到了最后一段时间，大部分时间都处于停机状态，无法处理小体积，也无法达到分离小分子所需的高速。拟建的装置将为高价值营养食品(鱼油、omega-3脂肪酸、蛋白质、氨基酸)的分离提供充足的离心力；药物(生物活性多肽、果糖霉素、胰凝乳酶、胃酶、胶原酶、酶抑制剂：α-1抗蛋白酶、α-2巨球蛋白、抗凝剂：肝素和类肝素糖胺聚糖)的分离。只有在较高的离心力下，才能提取酶抑制剂(α-1抗蛋白酶、α-2巨球蛋白)和抗凝血剂(肝素和类肝素糖胺聚糖)并进行纯化和回收。不可能从鱼血中的其他脂蛋白中分离乳杆菌微粒(用于测量特定脂肪酸的稳定碳同位素)，也不可能提取和纯化由天然生物聚合物壳聚糖、细菌细胞中的抗生素(杰霉素)和腺苷和类黄酮的纳米胶囊形成的纳米颗粒。将从鱼类废物和藻类中分离出脂肪，以确定鱼油和生物燃料的理论产量，这将决定生产鱼油所需的鱼类废物的数量和微藻生产操作的规模。拟议的仪器将用于几个研究项目，这些项目利用可再生自然资源(藻类)、废物(鱼类和动物提取物)生产高附加值产品(生物柴油、营养食品、药品)，并缓解与处理这些废物有关的环境问题，从而提高现有产业的可持续性，创造新的产业和新的就业机会，使加拿大在环境、生物能源和生物医药技术方面处于领先地位。

The requested Micro-Ultra Centrifuge is very effective in pelletizing subcellular particles, viruses, DNA and RNA, protein purification and isolation, differential flotation of lipoproteins, isolation of cellular organelles (ribosomes, mitochondria, microsomes) and nanoparticles. The unit is very crucial for Drs. Ghaly, Brooks, Budge, Ghanem, Gill and Yildiz research groups. The current unit is 46 years old and on its last leg, is down most of the time and does not handled small volumes nor the required high speed for the separation of small molecules. The proposed unit will provide ample centrifugal force for separation of high value nutraceuticals (fish oil, omega-3 fatty acids, proteins, aminoacids) pharmaceuticals (bioactive peptides, jadomycin, chymotrypsin, pepsin, collagenase, enzyme inhibitors: alpha-1 antiproteinase, alpha-2 macroglobulin, anticoagulants: heparin and heparin-like glycosaminoglycans). Extraction of enzyme inhibitors (alpha-1 antiproteinase, alpha-2 macroglobulin) and anticoagulants (heparin and heparin-like glycosaminoglycans) and their purification and recovery can only be possible at higher centrifugal force. It is impossible to carry out the separation of chylomicrons from other lipoproteins in fish blood (for measuring the stable carbon isotopes of specific fatty acids) or to extract and purify the nanoparticles formulated from the natural biopolymer chitosan, antibiotics (jadomycin) from bacteria cells and nanoencapsulation of adenosine and flavonoids. Lipids from fish waste and algae will be separated for determining the theoretical fish oil and biofuel yield which will determine the amount of fish waste required for fish oil production and the size of microalgae production operation. The proposed instrument will be use in several research projects which utilize renewable natural resources (algae), waste (fish and animal rendering) to produce high value added products (biodiesel, nutraceuticals, pharmaceuticals) and mitigate environmental problems associated with the disposal of these wastes, thereby improving the sustainability of current industries, creating new industries and new employment opportunities and putting Canada in the fore front of environmental, bioenergy and biomedical technology.