Recombinant cystatins in plants

植物中的重组半胱氨酸蛋白酶抑制剂

• 批准号: 194487-2010
• 负责人: Michaud, Dominique
• 金额: $ 3.64万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=514030
• 关键词: Recombinant; cystatins; plants

Several studies documented the usefulness of recombinant protease inhibitors for the development of insect-resistant transgenic crops, but a number of recent studies also described unintended effects of these proteins modifying the host plant's metabolic characteristics. Based on novel experimental approaches involving functional proteomics and transient protein expression, this project will aim at characterizing further the effects of protease inhibitors in planta, and at identifying the enzymes targeted by these inhibitors. Genetically engineered protease inhibitor variants with different affinities for the host plant's target enzymes will then be selected to test the feasibility of avoiding, or by contrast amplifying, the unintended effects observed. From a practical viewpoint, the general goal of this project, which is not only to identify the endogenous targets of a foreign protein in planta but also to modulate its resulting effects, will represent a first 'proof-of-concept' of our ability to rationally controlling the fine balance between the intended and unintended effects of a biologically-active foreign protein expressed in genetically modified crops.

一些研究证明了重组蛋白酶抑制剂对于开发抗虫转基因作物的有用性，但最近的一些研究也描述了这些蛋白质改变宿主植物代谢特征的意外影响。基于新的实验方法，涉及功能蛋白质组学和瞬时蛋白质表达，该项目的目的是进一步表征蛋白酶抑制剂在植物中的作用，并确定这些抑制剂靶向的酶。然后将选择对宿主植物的靶酶具有不同亲和力的基因工程蛋白酶抑制剂变体，以测试避免或通过对比放大观察到的非预期效应的可行性。从实际的角度来看，这个项目的总体目标，这不仅是确定外源蛋白在植物中的内源性目标，而且还调节其产生的影响，将代表我们的能力，合理地控制的预期和非预期的影响之间的微妙平衡的生物活性外源蛋白在转基因作物中表达的第一个“概念验证”。