Functional characterization and regulation of pumilio proteins

Pumilio 蛋白的功能表征和调控

• 批准号: 355768-2009
• 负责人: Chua, Gordon
• 金额: $ 2.91万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=515191
• 关键词: Functional; characterization; regulation; pumilio; proteins

Posttranscriptional gene regulation determines the amount of protein produced by affecting mRNA stability and translation. This process is essential for cells to adapt and survive in changing environments. The Pumilio (Puf) protein family are key regulators that interact directly with a subset of mRNAs to induce their turnover and repress their translation. However, our understanding of how these proteins function remains limited. Our goal is to apply a powerful combination of genetics, cell biology, biochemistry and functional genomics in the fission yeast model system to elucidate Puf protein function in specific environmental conditions by the determination of their mRNA targets, mode of posttranscriptional control and protein partners. Our view is that this research will lead to novel mechanistic insights in the posttranscriptional regulation of genes in the cell's response to the environment. Because the function of genes is highly conserved from yeast to humans, these studies should be relevant in approach and content to human biology.

转录后基因调控通过影响mRNA的稳定性和翻译来决定蛋白质的产生量。这个过程对于细胞在不断变化的环境中适应和生存至关重要。 Pumilio（Puf）蛋白家族是直接与mRNA子集相互作用以诱导其周转并抑制其翻译的关键调节因子。 然而，我们对这些蛋白质功能的了解仍然有限。 我们的目标是应用遗传学，细胞生物学，生物化学和功能基因组学在裂变酵母模型系统的强大组合，以阐明Puf蛋白在特定环境条件下的功能，通过确定其mRNA的目标，转录后控制模式和蛋白质伴侣。 我们的观点是，这项研究将导致新的机制的见解，在转录后调控基因在细胞的环境反应。 由于基因的功能从酵母到人类都是高度保守的，这些研究在方法和内容上应该与人类生物学相关。