Bacteriophage production using self-cycling fermentation technology

利用自循环发酵技术生产噬菌体

• 批准号: 6398-2008
• 负责人: Cooper, David
• 金额: $ 2.03万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=520918
• 关键词: Bacteriophage; production; using; self; cycling

There is an increasing interest in the production of bacteriophages (phages), which can be used directly in phage therapy, diagnostics and as antibacterial agents and can also be used to produce recombinant proteins, which can be used as pharmaceuticals and in agriculture and food applications. The large scale production of phages is difficult. Self-cycling fermentation (SCF) was developed in my laboratories and is a method of growing microbes continuously, in synchronous culture. The work proposed here is an adaptation of SCF to significantly improve the production of phages. The research will apply a cycling strategy to the production of phages and, eventually, recombinant proteins having phages as vectors and adopt a novel control strategy which simplifies SCF and optimizes the production. The system will be developed as two independently cycling bioreactors. The first stage is used for the growth of the host and operates as a normal self-cycling fermentor (SCF). The second stage is used for the infection of the host by the phage and is called the self-cycling infection chamber (SCIC).

人们对噬菌体（噬菌体）的生产越来越感兴趣，它可以直接用于噬菌体治疗、诊断和抗菌剂，也可以用于生产重组蛋白，可用于药物和农业和食品应用。大规模生产噬菌体是困难的。自循环发酵（SCF）是在我的实验室开发的，是一种在同步培养中连续生长微生物的方法。本文提出的工作是对SCF的适应，以显着提高噬菌体的产生。本研究将把循环策略应用于噬菌体的生产，最终以噬菌体为载体的重组蛋白的生产，并采用一种新的控制策略来简化SCF并优化生产。该系统将由两个独立循环的生物反应器组成。第一阶段用于宿主的生长，并作为正常的自循环发酵罐（SCF）运行。第二阶段用于噬菌体对宿主的感染，称为自循环感染室（SCIC）。