Mutagenomics - Bridging Chemistry and Biology

突变基因组学 - 连接化学和生物学

• 批准号: 155155-2013
• 负责人: Loppnow, Glen
• 金额: $ 2.48万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=522568
• 关键词: Mutagenomics; Bridging; Chemistry; Biology

A major goal of our research program is to measure and understand the molecular-level damage mechanisms in nucleic acids. Our approach combines the development of novel tools and massively-parallel platforms for measuring molecular-scale nucleic acid damage, with elucidation of reaction mechanisms. We use primarily fluorescence, absorption and resonance Raman to characterize nucleic acid damage in detail, starting with the femtosecond excited-state structural dynamics. Our primary research interests include probing the deleterious effects of various damage insults on DNA and RNA on a genome-wide scale and understanding the sequence and structural determinants of such damage.

我们研究计划的一个主要目标是测量和理解核酸分子水平的损伤机制。我们的方法结合了新的工具和并行平台的发展，用于测量分子尺度的核酸损伤，并阐明了反应机制。我们主要使用荧光，吸收和共振拉曼来详细描述核酸损伤，从飞秒激发态结构动力学。我们的主要研究兴趣包括在全基因组范围内探索各种损伤对DNA和RNA的有害影响，并了解这种损伤的序列和结构决定因素。