Determinants of retinal generators and modulation of retinal output signal

视网膜发生器的决定因素和视网膜输出信号的调制

• 批准号: 171141-2009
• 负责人: Tremblay, Francois
• 金额: $ 1.82万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=522851
• 关键词: Determinants; retinal; generators; modulation; output

The electroretinogram (ERG) is an old clinical investigation tool developed in the 1950's that has recently regained momentum in basic and clinical sciences as an adjunct exploratory tool to genetically modified animal models. It is a complex composite evoked biopotential elicited at the level of the retina by a visual stimulus. We thus propose to study the interplay of the complex retinal generators after specific neuronal circuits have been pharmacologically dissociated, in several animal species commonly used in research and for different modalities of ERGs. This will be the basis of the development of ERG model for the swine, guinea pig and the rat, which all have different ERG characteristics more of less similar to human ones. Furthermore, we will study how the changes in the ERG signal translate into modifications of the retinal output (spike trains characteristics of retinal ganglion cells). We also propose to study the impact of anesthesia on the neural processing with the retina as the model, as this is a very neglected field of research that has a major impact on our interpretation of animal results. Finally, we want to quantify the waveform of the ERG signal by wavelet analysis, as we believe this contains useful information not detected by looking at the traditional amplitude and implicit time parameters. The research program proposed will take place in a rich multidisciplinary environment and will offer training opportunities that will link clinical and basic sciences.

视网膜电图（ERG）是在20世纪50年代开发的旧的临床研究工具，其最近在基础和临床科学中作为转基因动物模型的辅助探索工具重新获得动力。它是由视觉刺激在视网膜水平引起的复杂复合诱发生物电位。因此，我们建议研究特定的神经元回路已被分离后，复杂的视网膜发电机的相互作用，在研究中常用的几种动物和不同形式的ERG。 这将为建立猪、豚鼠和大鼠视网膜电图模型奠定基础，因为猪、豚鼠和大鼠都具有与人类或多或少相似的不同视网膜电图特征。 此外，我们将研究ERG信号的变化如何转化为视网膜输出的改变（视网膜神经节细胞的棘波序列特征）。 我们还建议以视网膜为模型研究麻醉对神经处理的影响，因为这是一个非常被忽视的研究领域，对我们解释动物结果有重大影响。 最后，我们希望通过小波分析来量化ERG信号的波形，因为我们相信这包含了通过查看传统的幅度和隐式时间参数无法检测到的有用信息。 拟议的研究计划将在丰富的多学科环境中进行，并将提供将临床和基础科学联系起来的培训机会。