Effect of selected antibiotics and NSAIDs on nitrogen cycling in wastewater environments

选定的抗生素和非甾体抗炎药对废水环境中氮循环的影响

• 批准号: 227565-2013
• 负责人: Gilbride, Kimberley
• 金额: $ 2.19万
• 依托单位: Ryerson University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=524018
• 关键词: Effect; selected; antibiotics; NSAIDs; nitrogen

Resident microbial populations are an integral part of the wastewater secondary treatment process and degrade many of the complex organic compounds thereby reducing the biological oxygen demand (BOD) of the wastewater. Nitrogen (ammonia) must be removed from the wastewater since high concentrations of ammonia can be toxic and low concentrations can cause the system to stop functioning. Release of effluent containing nitrogen can cause eutrophication of the lakes and streams it is released into. Previous research has acknowledged the vast diversity of microbes in the microbial communities and has identified many of the organisms in these systems. Ammonia oxidation is carried out by ammonia-oxidizing bacteria (AOB) and recently by a second group, ammonia-oxidizing archaea (AOA). This proposal will help to understand the significance of this functional redundancy. Wastewater samples and reactors containing wastewater effluent will be sampled and subjected to chemical and molecular analysis to determine the relative abundances of each of the groups and the corresponding activity of each group. Phylogenetic shifts of AOA and AOB have been seen in soils to be caused by different environmental parameters, therefore, the activity of each group will be assessed in the presence of the antibiotics and NSAIDS, contaminants that are detected more and more frequently in municipal sewage waste streams. This research will serve to advance our knowledge of AOA in wastewater system and their role in ammonia oxidation in pharmaceutical contaminated wastewater. The apparent redundancy of ammonia oxidizers may be necessary to maintain nitrification under changing conditions seen by municipal wastewater plants.

常驻微生物种群是废水二级处理过程的组成部分，并降解许多复杂的有机化合物，从而降低废水的生物需氧量（BOD）。必须从废水中去除氮（氨），因为高浓度的氨可能是有毒的，低浓度的氨可能导致系统停止运行。 含氮污水的排放会导致湖泊和溪流的富营养化。 以前的研究已经认识到微生物群落中微生物的巨大多样性，并确定了这些系统中的许多生物体。氨氧化由氨氧化细菌（AOB）进行，最近由第二组氨氧化古菌（AOA）进行。 这一建议将有助于理解这种功能冗余的重要性。 将对废水样品和含有废水流出物的反应器进行取样，并进行化学和分子分析，以确定各组的相对丰度和各组的相应活性。 AOA和AOB的系统发生变化已被视为在土壤中由不同的环境参数引起的，因此，每个组的活性将在抗生素和NSAIDS的存在下进行评估，这些污染物在城市污水废水流中越来越频繁地被检测到。 本研究将有助于提高我们对废水系统中AOA及其在制药废水氨氧化中的作用的认识。 氨氧化剂的明显冗余可能是必要的，以保持城市污水处理厂所看到的变化条件下的硝化作用。