Effect of selected antibiotics and NSAIDs on nitrogen cycling in wastewater environments

选定的抗生素和非甾体抗炎药对废水环境中氮循环的影响

• 批准号: 227565-2013
• 负责人: Gilbride, Kimberley
• 金额: $ 2.19万
• 依托单位: Ryerson University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629638
• 关键词: Effect; selected; antibiotics; NSAIDs; nitrogen

Resident microbial populations are an integral part of the wastewater secondary treatment process and degrade many of the complex organic compounds thereby reducing the biological oxygen demand (BOD) of the wastewater. Nitrogen (ammonia) must be removed from the wastewater since high concentrations of ammonia can be toxic and low concentrations can cause the system to stop functioning. Release of effluent containing nitrogen can cause eutrophication of the lakes and streams it is released into. Previous research has acknowledged the vast diversity of microbes in the microbial communities and has identified many of the organisms in these systems. Ammonia oxidation is carried out by ammonia-oxidizing bacteria (AOB) and recently by a second group, ammonia-oxidizing archaea (AOA). This proposal will help to understand the significance of this functional redundancy. Wastewater samples and reactors containing wastewater effluent will be sampled and subjected to chemical and molecular analysis to determine the relative abundances of each of the groups and the corresponding activity of each group. Phylogenetic shifts of AOA and AOB have been seen in soils to be caused by different environmental parameters, therefore, the activity of each group will be assessed in the presence of the antibiotics and NSAIDS, contaminants that are detected more and more frequently in municipal sewage waste streams. This research will serve to advance our knowledge of AOA in wastewater system and their role in ammonia oxidation in pharmaceutical contaminated wastewater. The apparent redundancy of ammonia oxidizers may be necessary to maintain nitrification under changing conditions seen by municipal wastewater plants.

常驻微生物种群是废水二级处理过程中不可或缺的一部分，可以降解许多复杂的有机化合物，从而降低废水的生物需氧量 (BOD)。必须从废水中去除氮（氨），因为高浓度的氨可能有毒，低浓度的氨可能导致系统停止运行。含氮废水的排放可能会导致排放到其中的湖泊和溪流发生富营养化。先前的研究已经承认微生物群落中微生物的巨大多样性，并鉴定了这些系统中的许多生物体。氨氧化是由氨氧化细菌（AOB）进行的，最近是由第二组氨氧化古菌（AOA）进行的。该建议将有助于理解这种功能冗余的重要性。对废水样品和含有废水流出物的反应器进行取样并进行化学和分子分析，以确定每个组的相对丰度以及每个组的相应活性。土壤中 AOA 和 AOB 的系统发育变化是由不同的环境参数引起的，因此，将在抗生素和 NSAIDS 存在的情况下评估每个组的活性，这些污染物在城市污水废物流中越来越频繁地检测到。这项研究将有助于增进我们对废水系统中 AOA 及其在药物污染废水中氨氧化中的作用的了解。氨氧化剂的明显冗余可能是在城市污水处理厂所见的不断变化的条件下维持硝化作用所必需的。