Genetic and cell biological dissection of pectin production in the mucilage secretory cells of Arabidopsis thaliana

拟南芥粘液分泌细胞果胶生产的遗传和细胞生物学解剖

• 批准号: 283250-2009
• 负责人: Western, Tamara
• 金额: $ 2.26万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=525651
• 关键词: Genetic; cell; biological; dissection; pectin

Plant cells are surrounded by a semi-rigid wall. This cell wall plays a critical role in almost all aspects of plant existence, ranging from plant growth, to cell and tissue cohesion, to interactions between cells and their environment. Thus, it is critical to understand the structure, production and modification of cell walls. Plant cell walls are primarily composed of polysaccharides: cellulose microfibrils are crosslinked via hemicellulose chains and embedded in a stiff gel of pectins. While cellulose is well-known for its structural role in determining the strength of the wall, pectins play key roles in cell-cell adhesion and modulating the fluidity and porosity of the wall. My long-term objective is to understand not only how pectins are synthesized and transported to the cell exterior, but also how these processes are regulated at the cellular level. Insight from my research provides valuable information that will contribute to the Canadian bio-based economy, particularly plant cell wall-based industrial, energy and agricultural products (e.g. wood, fibres, paper).

植物细胞被半刚性的壁包围。这种细胞壁在植物生存的几乎所有方面都起着关键作用，从植物生长到细胞和组织的凝聚力，再到细胞与环境之间的相互作用。因此，了解细胞壁的结构、产生和修饰至关重要。植物细胞壁主要由多糖组成：纤维素微纤丝通过半纤维素链交联并嵌入果胶的硬凝胶中。虽然纤维素以其在决定壁的强度中的结构作用而闻名，但果胶在细胞-细胞粘附和调节壁的流动性和孔隙率中起关键作用。我的长期目标是不仅要了解果胶是如何合成和运输到细胞外部的，而且还要了解这些过程是如何在细胞水平上进行调节的。我的研究提供了宝贵的信息，将有助于加拿大的生物经济，特别是植物细胞壁为基础的工业，能源和农产品（如木材，纤维，纸张）。