Role of Nod Proteins in Regulation of Intestinal Goblet Cell Biology in Enteric Parasitic Infection

Nod 蛋白在肠道寄生虫感染中肠道杯状细胞生物学调节中的作用

• 批准号: 311764-2013
• 负责人: Khan, Waliul
• 金额: $ 2.62万
• 依托单位: McMaster University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=526243
• 关键词: Role; Nod; Proteins; Regulation; Intestinal

Goblet cells are present throughout the gastrointestinal (GI) tract and are the main source of mucins in the gut. Mucus layer containing mucins forms a protective blanket and represents the front line of defense in the GI tract. Mucins are considered to play an important role in host protection in a number of enteric infections, including many parasitic infections. Gaining precise knowledge on goblet cell biology and mucin production is critical in understanding intestinal defense mechanism against various infectious agents. Gut flora are the collection of microorganisms that live within our GI tract and are predominantly made up of bacteria. Nod proteins are innate immune receptors and important role in recognition of bacterial infection through their detection of a component of the bacterial cell wall called peptidoglycan. In this application I propose to examine a model that links parasitic infection to gut flora and innate immune receptors to extend our understanding of intestinal goblet cell biology and mucin production. This grant will explore the idea that Nod proteins play an important role in the regulation of intestinal goblet cell biology, and enteric parasite infection influences goblet cell function by changing the composition of gut flora and influencing gut flora-Nod axis. Expression and function of Nod proteins in goblet cells and the role of gut flora-Nod axis in regulation of goblet cell biology in an enteric infection will be investigated by utilizing mucin producing cell line and parasite infected animals.

杯状细胞存在于整个胃肠道中，是肠道中粘蛋白的主要来源。 含有粘蛋白的粘液层形成一层保护毯，代表胃肠道的前线防御。 粘蛋白被认为在许多肠道感染（包括许多寄生虫感染）的宿主保护中发挥重要作用。获得有关杯状细胞生物学和粘蛋白产生的精确知识对于了解针对各种感染因子的肠道防御机制至关重要。肠道菌群是生活在胃肠道内的微生物的集合，主要由细菌组成。 Nod 蛋白是先天免疫受体，通过检测细菌细胞壁的肽聚糖成分，在识别细菌感染方面发挥着重要作用。 在本申请中，我建议研究一种将寄生虫感染与肠道菌群和先天免疫受体联系起来的模型，以扩展我们对肠道杯状细胞生物学和粘蛋白产生的理解。 该资助将探讨Nod蛋白在肠道杯状细胞生物学调节中发挥重要作用的观点，肠道寄生虫感染通过改变肠道菌群的组成和影响肠道菌群-Nod轴来影响杯状细胞的功能。将通过利用产生粘蛋白的细胞系和寄生虫感染的动物来研究Nod蛋白在杯状细胞中的表达和功能以及肠道菌群-Nod轴在肠道感染中杯状细胞生物学调节中的作用。