Simulation studies of nutraceuticals and antimicrobials in exotic environments

异国环境中营养保健品和抗菌药物的模拟研究

• 批准号: 312549-2009
• 负责人: Tomberli, Bruno
• 金额: $ 1.31万
• 依托单位: Brandon University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=526571
• 关键词: Simulation; studies; nutraceuticals; antimicrobials; exotic

Naturally occurring bacteria protect themselves by the formation of a sugary layer called a biofilm which allows them to resist to traditional cleansing techniques. Cleansing equipment "fouled" with biofilms is a major cost in many industries such as pulp and paper production, food processing and soil remediation. The ability of supercritical carbon dioxide (SC-CO2) to kill bacteria is documented but not well understood. Supercritical carbon dioxide is simply carbon dioxide (present in our breath and making up about 0.04% of the atmosphere) at pressures higher than 73 atmospheres and temperatures higher than 31 Celsius. Supercritcal carbon dioxide has properties that are useful to sterilization. The gas-like properties of SC-CO2 allow it to readily penetrate to the bacteria. Its liquid-like properties allow a much higher concentration of carbon dioxide to reach the bacteria compared to the gas. This project will explore how SC-CO2 kills bacteria using advanced theoretical techniques combined with computer simulation and determine if SC-CO2's antimicrobial capability can be further enhanced with small amounts of specially selected bactericidal molecules. Two types of additive will be studied: 1) short bactericidal proteins and 2) antioxidants. Because little or no theoretical modelling of bacterial membranes in SC-CO2 has been done, this research program is likely to yield novel results that may have a positive economic impact on industries affected by bacterial biofilm "fouling".

自然产生的细菌通过形成一种称为生物膜的糖层来保护自己，这使它们能够抵抗传统的清洁技术。 清洁被生物膜“污染”的设备是许多行业的主要成本，例如纸浆和纸张生产、食品加工和土壤修复。超临界二氧化碳（SC-CO2）杀死细菌的能力有文献记载，但尚未得到很好的理解。 超临界二氧化碳只是二氧化碳（存在于我们的呼吸中，约占大气的0.04%），压力高于73个大气压，温度高于31摄氏度。 超临界二氧化碳具有对灭菌有用的特性。 SC-CO2的气体性质使其易于渗透到细菌中。与气体相比，它的液体性质允许更高浓度的二氧化碳到达细菌。该项目将探索SC-CO2如何使用先进的理论技术结合计算机模拟杀死细菌，并确定SC-CO2的抗菌能力是否可以通过少量特别选择的杀菌分子进一步增强。将研究两种类型的添加剂：1）短杀菌蛋白和2）抗氧化剂。 由于很少或没有理论模型的细菌膜在超临界CO2已经完成，这项研究计划可能会产生新的结果，可能会有积极的经济影响的行业受细菌生物膜“污垢”。