Novel contrast mechanisms and devices for imaging in neurobiology

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Amongst all current imaging modalities, optical microscopy is the only method capable of probing live tissue with cellular and subcellular resolution and is the only one with a broad range of molecular contrast mechanisms. This versatility comes from the fact that light interacts well with molecules and consequently, contrasts based on density, absorption, fluorescence, and other advanced optical interactions are possible. Imaging strategies based on novel contrast mechanisms can be developed and integrated into current technology to enhance a biologist's toolbox, or can be developed into new imaging devices to enable innovative applications in life sciences. Our group stands at the interface between optical technologies and biology, and develops new modalities and devices for applications in life sciences, in particular for neuroscience. For instance, we are developing an optically-sectioned neuronal activity imaging microscope with wide field excitation that permits very fast imaging even in thick tissue for fundamental studies in neurobiology. Also, we are developing imaging techniques based on laser technology that are complementary to techniques used today in medicine. For example, since brain biopsies are not possible, we are developing a micro endoscope for imaging deep brain structures with minimal invasiveness and colateral damage. Finally, we have developed a microscope that can image spinal cord and other tissues in live animals with a very high spatial resolution at video rates. We are integrating it with our optical techniques for routine use in biology.