Oxidative injury during the storage of cell-based biosensors and therapeutics

细胞生物传感器和治疗剂储存过程中的氧化损伤

• 批准号: 341754-2013
• 负责人: Acker, Jason
• 金额: $ 1.82万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=527447
• 关键词: Oxidative; injury; during; storage; cell

The development of preservation and long-term storage techniques is a critical requirement for the successful clinical and commercial use of cell-based technologies. With the performance of tissue engineering, cell and tissue transplantation, and transfusion medicine dependent on the quality of the living cells, preserving cells and tissues remains one of the most important challenges facing reparative medicine. In addition, the stability of cells or cell products in portable diagnostic tests under extreme environmental conditions (temperature and humidity) is considered to be a critical requirement for their use in accurately diagnosing disease in the developing world. There are a number of approaches to the preservation of cell-based technologies including refrigeration, freezing and dry storage (desiccation). Cell injury is related to how the cell responds to the physical and chemical changes that occur during the preservation processes. Over the past twenty years, enormous progress has been made understanding the response of natural systems (for example insects, plants, and nematodes) to environmental stress and the molecular strategies that are responsible for natural desiccation- and freeze-tolerance. Oxidative stress during preservation is the result of the accumulation of reactive oxygen species or loss of antioxidant capacity that must be accommodated if cell survival is to occur. Our long term goals are to understand and then mimic the native stress response strategies of natural systems to improve the preservation of cell-based biosensors and therapeutics. It is our hypothesis that improved methods

保存和长期储存技术的发展是基于细胞的技术成功临床和商业应用的关键要求。随着组织工程、细胞和组织移植以及输血医学的性能依赖于活细胞的质量，保存细胞和组织仍然是修复医学面临的最重要挑战之一。此外，极端环境条件（温度和湿度）下便携式诊断测试中的细胞或细胞产品的稳定性被认为是其用于准确诊断发展中国家疾病的关键要求。有许多方法可以保存基于细胞的技术，包括冷藏、冷冻和干燥储存（干燥）。细胞损伤与细胞对保存过程中发生的物理和化学变化的反应有关。在过去的二十年里，在理解自然系统（例如昆虫，植物和线虫）对环境胁迫的反应以及负责天然干燥和冷冻耐受性的分子策略方面取得了巨大进展。保存过程中的氧化应激是活性氧物质积累或抗氧化能力丧失的结果，如果细胞要存活，则必须适应这些结果。我们的长期目标是了解并模仿自然系统的天然应激反应策略，以改善基于细胞的生物传感器和治疗方法的保存。我们的假设是改进的方法