Ecotoxicological biomarker development and validation

生态毒理学生物标志物的开发和验证

• 批准号: 360557-2009
• 负责人: Holdway, Douglas
• 金额: $ 2.55万
• 依托单位: University of Ontario Institute of Technology
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=528097
• 关键词: Ecotoxicological; biomarker; development; validation

The overall objective of this research program is to develop, validate and apply a suite of non-lethal biomarkers of exposure to, and effects of, environmental contaminants in fish. This will be achieved by the undertaking the following tasks: Develop a species specific protocol for applying the innovative and new non-lethal biomarker technique, bioeletrical impedance analysis (BIA), to determining a rapid assessment of fish proximate body composition, specifically lipid and energy density. The BIA technique, which was developed for human health applications, instantaneously measures the resistance and reactance (or capacitance) along the body of the fish, which is then used to quantify body lipid and energy levels. Initial work will focus on creating calibration equations for rainbow trout (Oncorhynchus mykiss), Japanese medaka (Oryzias latipes) and Florida flagfish (Jordanella floridae). Validation will involve lipid and calorimetric measurements to be made using lethal sampling to develop appropriate mathematical models for each species. Application of non-lethal biomarker techniques will then be undertaken to assess the biochemical and bioenergetic impacts of toxicant exposure to fish in the laboratory. Correlations between altered blood enzyme biomarkers and bioenergetics will be made in relation to specific doses and time following exposure. Field biomonitoring using caged-fish populations held in regions of varying contaminant levels will be done to measure the bioenergetic costs of detoxification and acclimation over time. This novel information will result in increased understanding of the impacts of contaminants on the physiology and health of fish populations. Finally, calibration equations for a wider range of local freshwater fish species will be created to permit fish community bioenergetic studies to be undertaken. Thus, this research program will enhance efforts to rapidly assess condition of fish species in the wild as well as in laboratory situtations, and to permit more precise understanding of the bioenergetics of contaminant exposure on different spatial and temporal scales. Development and validation of this non-lethal BIA technique will increase our ability to manage important fish populaions for the benefit of all Canadians.

该研究计划的总体目标是开发、验证和应用一套非致命性生物标志物，以了解鱼类环境污染物的暴露及其影响。这将通过执行以下任务来实现： 制定物种特定协议，应用创新的新型非致命生物标记技术、生物电阻抗分析 (BIA)，来确定鱼类近端身体成分的快速评估，特别是脂质和能量密度。 BIA 技术是为人类健康应用而开发的，可即时测量鱼身体的电阻和电抗（或电容），然后用于量化身体脂质和能量水平。初步工作将集中于为虹鳟鱼 (Oncorhynchus mykiss)、日本青鳉鱼 (Oryzias latipes) 和佛罗里达旗鱼 (Jordanella floridae) 创建校准方程。验证将涉及使用致死采样进行脂质和热量测量，以便为每个物种开发适当的数学模型。然后将应用非致命生物标记技术来评估实验室中鱼类接触毒物的生化和生物能量影响。将根据暴露后的特定剂量和时间建立改变的血液酶生物标志物和生物能学之间的相关性。将使用不同污染物水平地区的网箱鱼群进行现场生物监测，以测量一段时间内解毒和驯化的生物能成本。这些新信息将加深人们对污染物对鱼类生理和健康影响的了解。最后，将为更广泛的当地淡水鱼类物种创建校准方程，以便进行鱼类群落生物能研究。因此，该研究计划将加强努力，快速评估野生和实验室条件下鱼类的状况，并更准确地了解不同空间和时间尺度上污染物暴露的生物能学。这种非致命性 BIA 技术的开发和验证将提高我们管理重要鱼类种群的能力，造福所有加拿大人。