Single-molecule investigations of the microtubule cytoskeleton

微管细胞骨架的单分子研究

• 批准号: 372593-2009
• 负责人: Brouhard, Gary
• 金额: $ 2.7万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=528551
• 关键词: Single; molecule; investigations; microtubule; cytoskeleton

Cells can build an amazing variety of structures from proteins, structures notable for their range of shapes, their ability to respond to stimulus, and their motility. My scientific interests are in the biophysical mechanisms by which cells engineer these large-scale structures---in other words, the molecular basis of morphology. The subject of this grant application is the microtubule cytoskeleton. Microtubules are long, slender filaments from which the cell constructs the mitotic spindle, axonemes, and the cytoskeleton. These structures are not static. Rather, they are broken down and rebuilt as the cell undergoes differentiation and progresses through the cell cycle. My aim is to understand the means by which cells accomplish these remarkable rearrangements of their microtubules.

细胞可以从蛋白质中构建出各种各样的结构，这些结构以其形状范围，对刺激的反应能力和运动性而闻名。我的科学兴趣是细胞工程这些大规模结构的生物物理机制-换句话说，形态学的分子基础。本基金申请的主题是微管细胞骨架。微管是细长的细丝，细胞由它构成有丝分裂纺锤体、轴丝和细胞骨架。这些结构不是静态的。相反，当细胞经历分化并在细胞周期中进展时，它们被分解和重建。我的目标是了解细胞是如何完成这些微管的显著重排的。