Automated, miniaturised pathogen detection systems

自动化、小型化病原体检测系统

• 批准号: 396882-2010
• 负责人: Backhouse, Christopher
• 金额: $ 16.9万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Strategic Projects - Group
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=529234
• 关键词: Automated; miniaturised; pathogen; detection; systems

We propose to develop economically viable genetic pathogen detection applications upon extremely inexpensive "lab on a chip" (LOC) systems that we have recently demonstrated. A central challenge for genetic diagnostics is not the testing capability itself, but the capability of cost-effective testing. Largely due to high labour and reagent costs, the impact of molecular biology upon water quality testing has to date been modest. We have assembled a team with a demonstrated track record in the miniaturization and co-adaptation of technologies for the transfer of flagship molecular biology procedures from conventional instrumentation to LOC systems. Our scientific objective is to move standard molecular biology onto a new and inexpensive LOC platforms - this will require co-adaptation and redesign of both the LOC technologies and the molecular biology technologies. Working with DALSA, a Canadian world leader in this arena, this project would generate a new technology for LOC use and place our public health partners in the vanguard of demonstrating the powerful benefits of these new technologies, while providing a market opportunity for our industrial partner. A challenge faced by industry and government alike is that of ascertaining the presence of pathogens in our water. This will require sensitive, selective and inexpensive systems. We propose to apply advanced lab-on-chip technologies to detect sentinel pathogens, i.e. pathogens that are known to be correlated with human health, and to do this with rapid and inexpensive systems that are capable of portable deployment.

我们建议开发经济上可行的遗传病原体检测应用程序上非常便宜的“芯片上的实验室”（ESTA）系统，我们最近已经证明。基因诊断的核心挑战不是检测能力本身，而是成本效益高的检测能力。主要由于劳动力和试剂成本高，分子生物学对水质检测的影响迄今为止一直不大。我们组建了一个团队，该团队在小型化和共同适应技术方面有着良好的记录，这些技术用于将旗舰分子生物学程序从传统仪器转移到生物系统。我们的科学目标是将标准分子生物学转移到一个新的和廉价的生物技术平台上-这将需要生物技术和分子生物学技术的共同适应和重新设计。与加拿大在这一竞技场的世界领导者DALSA合作，该项目将产生一种可供重复使用的新技术，并使我们的公共卫生合作伙伴成为展示这些新技术强大益处的先锋，同时为我们的工业合作伙伴提供市场机会。工业界和政府都面临着一个挑战，那就是确定我们的水中是否存在病原体。这将需要灵敏、有选择性和廉价的系统。我们建议应用先进的芯片实验室技术来检测前哨病原体，即已知与人类健康相关的病原体，并使用能够便携式部署的快速廉价系统来实现这一目标。