Probiotic bioactive for improvement of animal health and control of foodborne infections

益生菌具有生物活性，可改善动物健康和控制食源性感染

• 批准号: 452272-2013
• 负责人: Griffiths, Mansel
• 金额: $ 11.73万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=540748
• 关键词: Probiotic; bioactive; improvement; animal; health

The presence of pathogens in the food supply remains a major concern to consumers. Recent major foodborne disease outbreaks in Canada involving products of animal origin have prompted government agencies and all sectors of the food industry to focus on effective strategies to control the presence of pathogens at all points along the food chain. The present project involves an innovative strategy using natural bioactive molecules produced by probiotic bacteria to mitigate the impact of animal and human pathogens. Bioactive molecules produced by Lactobacillus acidophilus LA-5, and other probiotic bacteria, reduces the virulence of pathogens such as E. coli O157:H7 and Salmonella Typhimurium by switching off genes involved in adherence to epithelial cells of the gastrointestinal tract. The commercialization of such a product will help producers to improve the health of animals such as piglets and chickens. Application of the probiotic bioactive will also help to reduce carriage and fecal shedding of potential foodborne pathogens such as Salmonella, Clostridium perfringens and enterotoxigenic E. coli (ETEC) by preventing them from colonizing their animal hosts. This proposal seeks i) to evaluate the efficacy of the bioactives in animals, ii) to determine their effect on a number of other foodborne bacterial and viral pathogens, iii) to elucidate the mechanism whereby they exert their effect and iv) explore methods for their large scale production. The application of the bioactives in animal production could help to reduce the presence of foodborne pathogens in food as well as limit the use of antibiotics for the treatment of animal diseases.

食品供应中的病原体仍然是消费者的主要关注点。最近在加拿大爆发的涉及动物源性产品的重大食源性疾病促使政府机构和食品工业的所有部门关注有效的战略，以控制食物链沿着所有点的病原体的存在。本项目涉及一种创新战略，利用益生菌产生的天然生物活性分子来减轻动物和人类病原体的影响。由嗜酸乳杆菌LA-5和其他益生菌产生的生物活性分子降低了病原体如大肠杆菌的毒性。大肠杆菌O 157：H7和鼠伤寒沙门氏菌通过关闭参与粘附到胃肠道上皮细胞的基因。这种产品的商业化将有助于生产者改善仔猪和鸡等动物的健康。益生菌生物活性剂的应用还将有助于减少潜在的食源性病原体如沙门氏菌、产气荚膜梭菌和肠致病性大肠杆菌的携带和粪便脱落。大肠杆菌（ETEC），防止它们定植于动物宿主。该提案旨在i）评估生物活性物质在动物中的功效，ii）确定其对许多其他食源性细菌和病毒病原体的影响，iii）阐明其发挥作用的机制，iv）探索其大规模生产的方法。生物活性物质在动物生产中的应用有助于减少食品中食源性病原体的存在，并限制抗生素在动物疾病治疗中的使用。