Bioanalytical techniques and affinity assays for DNA damage and molecular interactions

DNA 损伤和分子相互作用的生物分析技术和亲和力测定

• 批准号: 186118-2013
• 负责人: Le, Chris
• 金额: $ 6.12万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=546030
• 关键词: Bioanalytical; techniques; affinity; assays; DNA

This research program will focus on developing analytical techniques and ultrasensitive assays for analysis of minute amounts of DNA damage and for studying interactions involving G-protein-coupled receptors (GPCRs). A variety of endogenous and environmental agents can damage human DNA, leading to various adverse health outcomes. Thus, it is crucial to detect DNA damage. Current immunoassays using antibodies cannot detect multiple types of DNA damage. The proposed research will use DNA repair proteins to recognize and bind to multiple types of DNA damage. Combined with capillary electrophoresis separation and laser induced fluorescence detection, the new affinity assays will enable detection of a wide range of DNA damage that is relevant to environmental, occupational, and clinical exposure, such as UV radiation, cancer treatment drugs, and other environmental carcinogens.

该研究计划将专注于开发分析技术和超灵敏度测定，用于分析微量DNA损伤和研究涉及G蛋白偶联受体（GPCR）的相互作用。各种内源性和环境因素可损害人类DNA，导致各种不良健康后果。因此，检测DNA损伤至关重要。目前使用抗体的免疫测定不能检测多种类型的DNA损伤。拟议的研究将使用DNA修复蛋白来识别和结合多种类型的DNA损伤。结合毛细管电泳分离和激光诱导荧光检测，新的亲和分析将能够检测与环境，职业和临床暴露相关的广泛的DNA损伤，如紫外线辐射，癌症治疗药物和其他环境致癌物。