Bioanalytical techniques and affinity assays for DNA damage and molecular interactions

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This research program will focus on developing analytical techniques and ultrasensitive assays for analysis of minute amounts of DNA damage and for studying interactions involving G-protein-coupled receptors (GPCRs). A variety of endogenous and environmental agents can damage human DNA, leading to various adverse health outcomes. Thus, it is crucial to detect DNA damage. Current immunoassays using antibodies cannot detect multiple types of DNA damage. The proposed research will use DNA repair proteins to recognize and bind to multiple types of DNA damage. Combined with capillary electrophoresis separation and laser induced fluorescence detection, the new affinity assays will enable detection of a wide range of DNA damage that is relevant to environmental, occupational, and clinical exposure, such as UV radiation, cancer treatment drugs, and other environmental carcinogens.